A Mouse Model of Acute Cartilage Injury and Repair

Anne-Sophie Thorup; Francesco Dell'Accio; Suzanne E Eldridge

doi:10.1007/978-1-0716-2839-3_24

A Mouse Model of Acute Cartilage Injury and Repair

Methods Mol Biol. 2023:2598:337-344. doi: 10.1007/978-1-0716-2839-3_24.

Authors

Anne-Sophie Thorup¹, Francesco Dell'Accio², Suzanne E Eldridge³

Affiliations

¹ Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
² Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK. fdellaccio@gmail.com.
³ Centre for Experimental Medicine and Rheumatology, William Harvey Research Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK. suzanne.e.eldridge@gmail.com.

PMID: 36355303
DOI: 10.1007/978-1-0716-2839-3_24

Abstract

Chondral defects are common and disabling. The development of pharmacological approaches for cartilage repair requires the availability of in vivo models which are amenable for gain and loss of function and ideally to genetic modification. In this chapter, we describe a method to induce full-thickness cartilage defects which, in young DBA/1 mice, heal spontaneously, but fail to heal in C57BL/6 mice of the same age or in aged DBA/1 mice. This model (or variants) has been used for genetic screenings to identify genes associated to repair capacity, to study stem cells involved in cartilage repair, and to study the function of molecules involved in repair mechanisms.

Keywords: Cartilage; Joint injury; Mouse models; Osteoarthritis; Repair.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cartilage, Articular*
Disease Models, Animal
Mice
Mice, Inbred C57BL
Mice, Inbred DBA

Abstract

Publication types

MeSH terms

Grants and funding