Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes

Huan Meng; Clare L Thompson; Clarissa R Coveney; Angus K Wann; Martin M Knight

doi:10.1007/978-1-0716-2839-3_12

Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes

Methods Mol Biol. 2023:2598:157-176. doi: 10.1007/978-1-0716-2839-3_12.

Authors

Huan Meng¹, Clare L Thompson^{1

2}, Clarissa R Coveney^{3

4}, Angus K Wann³, Martin M Knight^{5

6}

Affiliations

¹ Centre for Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, UK.
² Centre for Predictive in vitro Models, Queen Mary University of London, London, UK.
³ Kennedy Institute of Rheumatology, Medical Sciences Division, University of Oxford, Oxford, UK.
⁴ Department of Human Evolutionary Biology, Harvard University, Cambridge, MA, USA.
⁵ Centre for Bioengineering, School of Engineering and Materials Science, Queen Mary University of London, London, UK. m.m.knight@qmul.ac.uk.
⁶ Centre for Predictive in vitro Models, Queen Mary University of London, London, UK. m.m.knight@qmul.ac.uk.

PMID: 36355291
DOI: 10.1007/978-1-0716-2839-3_12

Abstract

Primary cilia regulate and coordinate a variety of cell signaling pathways important in chondrocyte physiology and cartilage development, health, and disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofluorescence labeling, microscopy, and measurement of chondrocyte primary cilia.

Keywords: Chondrocyte; Confocal microscopy; Immunofluorescence; Primary cilia; Quantification; Super resolution.

MeSH terms

Cartilage, Articular* / metabolism
Chondrocytes* / metabolism
Cilia / metabolism
Signal Transduction