Immunogenicity of engineered probiotics expressing conserved antigens of influenza virus and FLIC flagellin against H9N2 AIVinfection in mice

Tian-Yang Hu; Yi-Bing Lian; Jia-Hao Qian; Yong-Lei Yang; Emad Beshir Ata; Rong-Rong Zhang; Chun-Wei Shi; Gui-Lian Yang; Hai-Bin Huang; Yan-Long Jiang; Jian-Zhong Wang; Xin Cao; NanWang; Yan Zeng; Wen-Tao Yang; Chun-Feng Wang

doi:10.1016/j.rvsc.2022.10.024

Immunogenicity of engineered probiotics expressing conserved antigens of influenza virus and FLIC flagellin against H9N2 AIVinfection in mice

Res Vet Sci. 2022 Dec 31:153:115-126. doi: 10.1016/j.rvsc.2022.10.024. Epub 2022 Oct 30.

Authors

Affiliations

¹ College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of the Ministry of Education, Jilin Agricultural University, Changchun, China.
² Parasitology and Animal Diseases Dep., Vet. Res. Institute, National Research Centre, 12622, Dokki, Cairo, Egypt.
³ College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of the Ministry of Education, Jilin Agricultural University, Changchun, China. Electronic address: yangwentao@jlau.edu.cn.
⁴ College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Microecology and Healthy Breeding, Jilin Provincial Engineering Research Center of Animal Probiotics, Key Laboratory of Animal Production and Product Quality Safety of the Ministry of Education, Jilin Agricultural University, Changchun, China. Electronic address: wangchunfeng@jlau.edu.cn.

PMID: 36351352
DOI: 10.1016/j.rvsc.2022.10.024

Abstract

Avian influenza virus (AIV)is easy to cause diseases in birds and humans.It causes great economic losses to the poultry farms and leads to public health problems. Using vaccines is the main approach to control the prevalence of AIV. In our previously published article, a recombinant Lactobacillus plantarum (L. plantarum) expressing the NP-M2 peptide ofH9N2 AIV was generated, and its protective effect was evaluated in a chicken model. In this study, the protective effect was estimated in mice model. Humoral and cellular immune response parameters were measured using flow cytometry adding to body weight loss, survival rate, virus load, and histopathological changes in the lung. The obtained results elucidated that, the recombinant L. plantarum can promote the activation of dendritic cells (DC), proliferation of T and B cells adding to eliciting protective secretory IgA (sIgA) and humeral IgG level in mice model. Accordingly, it could be used as a patent vaccine to control the AIV infection.

Keywords: FLIC flagellin; Influenza virus; Lactobacillus plantarum; Mucosal immunity.