Insights into the role of prechlorination in algae-laden raw water distribution process: Algal organic matter and microcystin-LR release, extracellular polymeric substances (EPS) aggregation, and pipeline biofilm communities

Abstract

Prechlorination routinely applied for the treatment of algae-laden raw water has received extensive attention due to its influence on water quality and aquatic microbes. In this study, prechlorination experiments with different doses were conducted in sets of model raw water distribution systems. With the elevated dose of chlorine and prolonged hydraulic retention time (HRT), the ratio of intact algal cells decreased, and the stability of water enhanced. Dissolved organic carbon (DOC) and nitrogen (DON) increased when chlorine dose elevated from 0 to 0.5 mg/L but decreased with elevations from 0.5 to 2.0 mg/L, while UV₂₅₄ showed a monotonically increasing tendency. DOC, DON and extracellular microcystin-LR increase initially and decrease thereafter with the prolonged HRT. Notably, the effects of prechlorination on extracellular polymeric substances aggregation behavior on pipe walls and microbial community composition was revealed, providing more profound understanding of the community dynamics in this engineered system. This study helped optimize strategies to improve the stability and efficiency of pretreatment of algae-laden water.

Keywords: Algal cell integrity; Algal organic matter (AOM) release; Extracellular polymeric substances (EPS) aggregation; Microbial community.