DEAD-box ATPases as regulators of biomolecular condensates and membrane-less organelles

Daan Overwijn; Maria Hondele

doi:10.1016/j.tibs.2022.10.001

DEAD-box ATPases as regulators of biomolecular condensates and membrane-less organelles

Trends Biochem Sci. 2023 Mar;48(3):244-258. doi: 10.1016/j.tibs.2022.10.001. Epub 2022 Nov 4.

Authors

Daan Overwijn¹, Maria Hondele²

Affiliations

¹ Biozentrum, University of Basel, Basel, Switzerland.
² Biozentrum, University of Basel, Basel, Switzerland. Electronic address: maria.hondele@unibas.ch.

PMID: 36344372
DOI: 10.1016/j.tibs.2022.10.001

Abstract

RNA-dependent DEAD-box ATPases (DDXs) are emerging as major regulators of RNA-containing membrane-less organelles (MLOs). On the one hand, oligomerizing DDXs can promote condensate formation 'in cis', often using RNA as a scaffold. On the other hand, DDXs can disrupt RNA-RNA and RNA-protein interactions and thereby 'in trans' remodel the multivalent interactions underlying MLO formation. In this review, we discuss the best studied examples of DDXs modulating MLOs in cis and in trans. Further, we illustrate how this contributes to the dynamic assembly and turnover of MLOs which might help cells to modulate RNA sequestration and processing in a temporal and spatial manner.

Keywords: RNA processing; RNA-dependent DEAD-box ATPases; biomolecular condensates; cellular compartmentalization.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases
Biomolecular Condensates*
DEAD-box RNA Helicases
Organelles*
RNA

Substances

Adenosine Triphosphatases
RNA
DEAD-box RNA Helicases