Theoretical basis and experimental verification for evaluating the distribution of engineered nanoparticles in water-oil system

Abstract

The distribution of nanoparticles between aqueous and organic phases is universally considered as the starting point in predicting the fate and bioavailability of engineered nanoparticles in the environment. However, the theoretical basis for determining the distribution of nanoparticles in the immiscible water-oil system remains unclear. Here, for the first time, theoretical calculations were conducted to illustrate the underlying mechanism. It was suggested that the distribution of nanoparticles was largely controlled by the surface charge, particle size and surface hydrophobicity, and the water-oil interface was not the favorable phase for nanoparticles until a size threshold (10 nm) was met and the particle surface became amphiphilic. The theoretical results were verified by the experimental approaches of different nanoparticles distributed in the water-octanol mixture. The neutralization of a charged surface led to enhanced distribution into octanol for hydrophobic nanoparticles (e.g., aqueous C₆₀), yet it had little effect on hydrophilic nanoparticles (e.g., fullerol). More nanoparticles were trapped at the water-oil interface when size grew larger (e.g., Ag-CIT and Au-CIT with citrate) and the surface rendered amphiphilic by polymeric coatings (e.g., Ag-PVP with polyvinylpyrrolidone), though larger hydrophobic nanoparticles like aqu-nC₆₀ tended to stay in the octanol. The surface charge and hydrophobicity may have an important impact on the path-dependent distribution of nanoparticles in water- octanol system. The mechanistic insights based on theoretical calculations and experimental approaches will facilitate the accurate prediction of the distribution of engineered nanoparticles in biological and environmental systems.

Keywords: Distribution; Engineered nanoparticles; Hydrophobicity; Theoretical calculation; Water-oil system.