Ketoprofen and aspirin removal by laccase immobilized on date stones

Osamah J Al-Sareji; Mónika Meiczinger; Jasim M Salman; Raed A Al-Juboori; Khalid S Hashim; Viola Somogyi; Miklós Jakab

doi:10.1016/j.chemosphere.2022.137133

Ketoprofen and aspirin removal by laccase immobilized on date stones

Chemosphere. 2023 Jan;311(Pt 2):137133. doi: 10.1016/j.chemosphere.2022.137133. Epub 2022 Nov 4.

Authors

Osamah J Al-Sareji¹, Mónika Meiczinger², Jasim M Salman³, Raed A Al-Juboori⁴, Khalid S Hashim⁵, Viola Somogyi², Miklós Jakab⁶

Affiliations

¹ Environmental Research and Studies Center, University of Babylon, Al-Hillah, Iraq; Sustainability Solutions Research Lab, Faculty of Engineering, University of Pannonia, Egyetem Str. 10, Veszprém H, 8200, Hungary. Electronic address: eng.osama.jaber@uobabylon.edu.iq.
² Sustainability Solutions Research Lab, Faculty of Engineering, University of Pannonia, Egyetem Str. 10, Veszprém H, 8200, Hungary.
³ Department of Biology, College of Science, University of Babylon, Al-Hillah, Iraq.
⁴ NYUAD Water Research Center, New York University-Abu Dhabi Campus, Abu Dhabi, P.O. Box 129188, Abu Dhabi, United Arab Emirates; Water and Environmental Engineering Research Group, Department of Built Environment, Aalto University, P.O. Box 15200, Aalto, FI-00076, Espoo, Finland.
⁵ School of Civil Engineering and Built Environment, Liverpool John Moores University, UK; Department of Environmental Engineering, College of Engineering, University of Babylon, Al-Hillah, Iraq.
⁶ Research Centre of Engineering Sciences, Department of Materials Sciences and Engineering, University of Pannonia, P.O. Box 158, H-8201 Veszprém, Hungary.

PMID: 36343736
DOI: 10.1016/j.chemosphere.2022.137133

Abstract

In recent years, enzymatic remediation/biocatalysis has gained prominence for the bioremediation of recalcitrant chemicals. Laccase is one of the commonly investigated enzymes for bioremediation applications. There is a growing interest in immobilizing this enzyme onto adsorbents for achieving high pollutant removal through simultaneous adsorption and biodegradation. Due to the influence of the biomolecule-support interface on laccase activity and stability, it is crucial to functionalize the solid carrier prior to immobilization. Date stone (PDS), as an eco-friendly, low-cost, and effective natural adsorbent, was utilized as a carrier for laccase (fungus Trametes versicolor). After activating PDS through chemical treatments, the surface area increased by thirty-six-fold, and carbonyl groups became more prominent. Batch experiments were carried out for ketoprofen and aspirin biodegradation in aqueous solutions. After six cycles, the laccase maintained 54% of its original activity confirmed by oxidation tests of 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). In addition, the storage, pH, and thermal stability of immobilized laccase on functionalized date stone (LFPDS) were found to be superior to that of free laccase, demonstrating its potential for ongoing applications. In the aqueous batch mode, this immobilized laccase system was used to degrade 25 mg L^-1 of ketoprofen and aspirin, resulting in almost complete removal within 4 h of treatment. This study reveals that agricultural wastes such as date stone can successfully be valorized through simple activation techniques, and the final product can be used as an adsorbent and substrate for immobilization enzyme. The high efficiency of the LFPDS in removing ketoprofen and aspirin highlights the potential of this technology for removing pharmaceuticals and merits its continued development.

Keywords: Adsorption; Agro-industrial wastes; Date stones; Immobilization; Laccase; Pharmaceutical removal.

MeSH terms

Aspirin
Enzymes, Immobilized / metabolism
Ketoprofen*
Laccase / metabolism
Trametes

Substances

Ketoprofen
Enzymes, Immobilized
Laccase
Aspirin