Smart data collection for CryoEM

Tristan Bepler; Andrew J Borst; Jonathan Bouvette; Giuseppe Cannone; Songye Chen; Anchi Cheng; Ao Cheng; Quanfu Fan; Fanis Grollios; Harshit Gupta; Meghna Gupta; Theo Humphreys; Paul T Kim; Huihui Kuang; Yilai Li; Alex J Noble; Ali Punjani; William J Rice; Carlos Oscar S Sorzano; Scott M Stagg; Joshua Strauss; Lingbo Yu; Bridget Carragher; Clinton S Potter

doi:10.1016/j.jsb.2022.107913

Smart data collection for CryoEM

J Struct Biol. 2022 Dec;214(4):107913. doi: 10.1016/j.jsb.2022.107913. Epub 2022 Oct 28.

Authors

Tristan Bepler¹, Andrew J Borst², Jonathan Bouvette³, Giuseppe Cannone⁴, Songye Chen⁵, Anchi Cheng¹, Ao Cheng⁶, Quanfu Fan⁷, Fanis Grollios⁸, Harshit Gupta⁹, Meghna Gupta¹⁰, Theo Humphreys¹¹, Paul T Kim¹, Huihui Kuang¹, Yilai Li¹², Alex J Noble¹, Ali Punjani¹³, William J Rice¹⁴, Carlos Oscar S Sorzano¹⁵, Scott M Stagg¹⁶, Joshua Strauss¹⁷, Lingbo Yu⁸, Bridget Carragher¹, Clinton S Potter¹⁸

Affiliations

¹ New York Structural Biology Center, New York, NY, USA.
² University of Washington, Institute for Protein Design, Seattle, WA, USA.
³ National Institute of Environmental Health Sciences, NIH, Durham, NC, USA.
⁴ Laboratory for Molecular Biology, Medical Research Council, Cambridge, England.
⁵ California Institute of Technology, Pasadena, CA, USA.
⁶ Northwestern University, Evanston, IL, USA.
⁷ MIT-IBM Watson AI Lab, Cambridge, MA, USA.
⁸ ThermoFisher Scientific, Eindhoven, The Netherlands.
⁹ SLAC National Accelerator Laboratory, Menlo Park, CA, USA.
¹⁰ University of California at San Francisco, San Francisco, CA, USA.
¹¹ Pacific Northwest CryoEM Center, Portland, OR, USA.
¹² University of Michigan, Ann Arbor, MI, USA.
¹³ Structura Biotechnology, Toronto, Canada.
¹⁴ New York University School of Medicine, New York, NY, USA.
¹⁵ Biocomputing Unit, Natl. Center of Biotechnology, CSIC, Madrid, Spain.
¹⁶ Florida State University, Tallahassee, FL, USA.
¹⁷ University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
¹⁸ New York Structural Biology Center, New York, NY, USA. Electronic address: cpotter@nysbc.org.

PMID: 36341954
DOI: 10.1016/j.jsb.2022.107913

Abstract

This report provides an overview of the discussions, presentations, and consensus thinking from the Workshop on Smart Data Collection for CryoEM held at the New York Structural Biology Center on April 6-7, 2022. The goal of the workshop was to address next generation data collection strategies that integrate machine learning and real-time processing into the workflow to reduce or eliminate the need for operator intervention.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Data Collection*

Grants and funding

P30 CA016086/CA/NCI NIH HHS/United States