CTAB enabled microwave-hydrothermal assisted mesoporous Zn-doped hydroxyapatite nanorods synthesis using bio-waste Nodipecten nodosus scallop for biomedical implant applications

Abstract

In biomedical exploration, the predominant characteristic is synthesizing and fabricating multifunctional nanostructure with intensified biocompatibility and excellent antibacterial applications to avoid post-surgical implant failure. The objective of the current study is to examine ideal mesoporous zinc-doped hydroxyapatite (HAp) for future use in the field of biomedical research. In the present investigation, we synthesized mesoporous Zn-doped HAp nanorods with varied mole concentrations using a profound microwave hydrothermal method utilizing bio-waste Nodipecten nodosus scallop as a calcium source and CTAB as an organic modifier. Bio-waste Nodipecten nodosus scallop is a widely available cheap calcium precursor which is converted into pure and zinc-doped hydroxyapatite nanorods with the help of the microwave hydrothermal method. Different analytical techniques like spectroscopy and electron microscopy were employed to evaluate and precisely characterize the structural and morphological characteristics in synthesized pure and mesoporous Zn-doped HAp nanorods. CTAB and microwave hydrothermal methods successfully create mesoporous Zn-doped hydroxyapatite nanorods with different sizes and morphology. Mesoporous Zinc-doped HAp nanorods show excellent antibacterial activity against Klebsiella pneumoniae (MTCC 7407) and Bacillus subtilis (MTCC 1133), compared to other nanorods. ZnHAp-3 shows notable excellent results of antibacterial effect towards K. pneumoniae and B. subtilis, by exhibiting 12.36 ± 0.12 and 13.12 ± 0.16 mm zone of inhibition. Furthermore, ZnHAp-1 shows the lower zone of inhibition, while the ZnHAp-3 sample shows the highest zone of inhibition. A foremost study performed was toxicity assays to validate safe attributes of mesoporous zinc-doped HAp intensified with the proliferation function of the zebrafish model. The results reveal the non-toxic behavior of pure and mesoporous zinc-doped HAp samples. Thus, our studies provide evidence for the synthesis technique for the mesoporous zinc-doped HAp nanorods using a novel CTAB-enabled microwave hydrothermal method utilizing bio-waste Nodipecten nodosus scallop as a calcium source will be alternative affordable biocidal antibacterial materials for controlling post-surgical implant failures.

Keywords: Antibacterial activity; Biomaterials; Calcium phosphate; Implant failure; Mesoporous nanorods; Zebrafish toxicity.