Biosensor Cell-Fit-HD4D for correlation of single-cell fate and microscale energy deposition in complex ion beams

Julian Schlegel; Hans Liew; Katrin Rein; Oleh Dzyubachyk; Jürgen Debus; Amir Abdollahi; Martin Niklas

doi:10.1016/j.xpro.2022.101798

Biosensor Cell-Fit-HD^4D for correlation of single-cell fate and microscale energy deposition in complex ion beams

STAR Protoc. 2022 Oct 29;3(4):101798. doi: 10.1016/j.xpro.2022.101798. eCollection 2022 Dec 16.

Authors

Julian Schlegel¹, Hans Liew², Katrin Rein², Oleh Dzyubachyk³, Jürgen Debus⁴, Amir Abdollahi⁴, Martin Niklas⁵

Affiliations

¹ Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, 69120 Heidelberg, Germany; National Center for Tumor Diseases, German Cancer Consortium, Heidelberg Institute of Radiation Oncology and National Center for Radiation Oncology, 69120 Heidelberg, Germany. Electronic address: j.schlegel@dkfz.de.
² Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, 69120 Heidelberg, Germany; National Center for Tumor Diseases, German Cancer Consortium, Heidelberg Institute of Radiation Oncology and National Center for Radiation Oncology, 69120 Heidelberg, Germany.
³ Department of Radiology and Department of Cell and Chemical Biology, Leiden University Medical Center, 2333 ZC Leiden, the Netherlands.
⁴ Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, 69120 Heidelberg, Germany; National Center for Tumor Diseases, German Cancer Consortium, Heidelberg Institute of Radiation Oncology and National Center for Radiation Oncology, 69120 Heidelberg, Germany; Heidelberg Ion-Beam Therapy Center (HIT), 69120 Heidelberg, Germany.
⁵ Division of Molecular and Translational Radiation Oncology and Clinical Cooperation Unit Translational Radiation Oncology, German Cancer Research Center (DKFZ) and Heidelberg University Hospital, 69120 Heidelberg, Germany; National Center for Tumor Diseases, German Cancer Consortium, Heidelberg Institute of Radiation Oncology and National Center for Radiation Oncology, 69120 Heidelberg, Germany. Electronic address: m.niklas@dkfz.de.

Abstract

We present a protocol for the biosensor Cell-Fit-HD^4D. It enables long-term monitoring and correlation of single-cell fate with subcellular-deposited energy of ionizing radiation. Cell fate tracking using widefield time-lapse microscopy is uncoupled in time from confocal ion track imaging. Registration of both image acquisition steps allows precise ion track assignment to cells and correlation with cellular readouts. For complete details on the use and execution of this protocol, please refer to Niklas et al. (2022).

Keywords: Biophysics; Biotechnology and bioengineering; Cancer; Microscopy; Molecular biology; Molecular/Chemical probes; Single cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Cell Tracking
Linear Energy Transfer*
Microscopy, Confocal / methods
Radiometry / methods