Large-Scale Complete Sequencing and Haplotyping of 1-10 kb DNA Molecules Using Short Massively Parallel Reads

Youtao Liu; Fei Fan; Radoje Drmanac; Brock A Peters; Ou Wang

doi:10.1007/978-1-0716-2819-5_4

Large-Scale Complete Sequencing and Haplotyping of 1-10 kb DNA Molecules Using Short Massively Parallel Reads

Methods Mol Biol. 2023:2590:59-70. doi: 10.1007/978-1-0716-2819-5_4.

Authors

Youtao Liu¹, Fei Fan², Radoje Drmanac³, Brock A Peters³, Ou Wang⁴

Affiliations

¹ MGI, Shenzhen, Guangdong Province, PR China.
² BGI-Shenzhen, Shenzhen, Guangdong Province, PR China.
³ Advanced Genomics Technology Laboratory, Complete Genomics/MGI, San Jose, CA, USA.
⁴ BGI-Shenzhen, Shenzhen, Guangdong Province, PR China. wangou@genomics.cn.

PMID: 36335492
DOI: 10.1007/978-1-0716-2819-5_4

Abstract

In this chapter, we describe a simple, low-cost method for making many copies of a single DNA molecule (1-10 kb in length) as a concatemer on a long DNA strand. This can enable applications requiring high-quality contiguous sequence and haplotype data from long single DNA molecules at large scale.

Keywords: Long-read sequencing; Single molecule sequencing; stLFR; stcLFR.

MeSH terms

DNA* / genetics
Haplotypes / genetics
High-Throughput Nucleotide Sequencing* / methods
Sequence Analysis, DNA / methods

Substances

DNA