Alleviation of aqueous nitrogen loss from paddy fields by growth and decomposition of duckweed (Lemna minor L.) after fertilization

Abstract

Runoff loss of nitrogen from paddy fields has received increasing attention in recent years. Duckweed is an aquatic plant frequently found in paddy fields. In this study, the effects of duckweed (Lemna minor L.) in floodwater on aqueous nitrogen losses from paddy fields were systematically investigated. Results demonstrated that the growth of duckweed decreased total nitrogen concentrations in floodwater and nitrogen runoff loss from paddy fields by 16.7%-18.3% and 11.2%-13.6%, respectively. Moreover, compared with NO₃^-, NH₄⁺ was preferentially removed by duckweed. ¹⁵N isotope tracer experiments revealed that the growth and decomposition of duckweed acted as a "buffer" against the nitrogen variation in floodwater after fertilization. During the growth of duckweed, leaves were found to be the principal organ to assimilate NH₄⁺ and release NO₃^- by using non-invasive micro-test technology. Duckweed degradation increased the content of hydrophobic acids and marine humic-like substances in floodwater, which promoted the migration of nitrogen from floodwater to soil. Redundancy analysis and structural equation models further illustrated that pH and temperature variation in floodwater caused by duckweed played a greater role in aqueous nitrogen loss reduction than the nitrogen accumulation in duckweed. This study suggested that the growth of duckweed in paddy fields was an effective supplementary method for controlling aqueous nitrogen loss during agricultural production.

Keywords: Duckweed; Floodwater; Nitrogen; Rice; Runoff loss.