Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus

Dalin He; Min Sun; Xiaoning Jiang; Shuai Zhang; Feng Wei; Bingrong Wu; Youxiang Diao; Yi Tang

doi:10.1016/j.jviromet.2022.114643

Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus

J Virol Methods. 2023 Jan:311:114643. doi: 10.1016/j.jviromet.2022.114643. Epub 2022 Nov 2.

Authors

Dalin He¹, Min Sun¹, Xiaoning Jiang¹, Shuai Zhang¹, Feng Wei¹, Bingrong Wu¹, Youxiang Diao², Yi Tang³

Affiliations

¹ College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong Province, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.
² College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong Province, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China. Electronic address: yxdiao@126.com.
³ College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong Province, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China. Electronic address: tyck288@163.com.

PMID: 36332715
DOI: 10.1016/j.jviromet.2022.114643

Abstract

Goose astrovirus (GAstV) characterized by articular and visceral gout, is an emerging pathogen with a wide distribution on mainland China, leading to serious economic losses in the goose-raising industry. Because vaccines to prevent GAstV infections are not available currently, early diagnosis is critical when treating symptomatic geese and in preventing GAstV transmission. In this context, a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) to detect GAstV-specific antibodies from geese was developed, and the detections were optimized. A series of experiments proved that the ic-ELISA shows excellent diagnostic performance and discriminatory power with high sensitivity and specificity. The ic-ELISA for GAstV detection was applied on 67 field serum samples, and comparing the detection results with the virus neutralization test verified the accuracy of the ic-ELISA. The correlation coefficient between the ic-ELISA and the virus neutralization test was 80%, demonstrating the proposed ic-ELISA method could be a useful and effective tool for the diagnostic, serological epidemiological investigation and immune monitoring of the GAstV in goose-producing regions.

Keywords: Antibodies; Diagnostic; GAstV; Goose; Serological; ic-ELISA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral
Astroviridae Infections* / diagnosis
Astroviridae Infections* / veterinary
Avastrovirus*
Enzyme-Linked Immunosorbent Assay / methods
Geese

Substances

Antibodies, Viral