CRISPR-assisted transposition: TnsC finds (and threads) the needle in the haystack

Amer A Hossain; Luciano A Marraffini

doi:10.1016/j.molcel.2022.10.014

CRISPR-assisted transposition: TnsC finds (and threads) the needle in the haystack

Mol Cell. 2022 Nov 3;82(21):3968-3969. doi: 10.1016/j.molcel.2022.10.014.

Authors

Amer A Hossain¹, Luciano A Marraffini²

Affiliations

¹ Laboratory of Bacteriology, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA.
² Laboratory of Bacteriology, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA; Howard Hughes Medical Institute, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA. Electronic address: marraffini@rockefeller.edu.

PMID: 36332604
DOI: 10.1016/j.molcel.2022.10.014

Abstract

Hoffmann et al. (2022) demonstrate that RNA-guided transposons are remarkably sequence specific due to the action of a AAA+ ATPase, TnsC, that recruits the transposase to the correct target site.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Clustered Regularly Interspaced Short Palindromic Repeats
DNA Transposable Elements / genetics
DNA, Bacterial
DNA-Binding Proteins / genetics
Escherichia coli / genetics
Escherichia coli Proteins* / genetics
Transposases / genetics
Transposases / metabolism

Substances

Escherichia coli Proteins
DNA Transposable Elements
DNA, Bacterial
DNA-Binding Proteins
Transposases