Molecular Surveillance of West Nile Virus in Mosquitoes and Sentinel Chickens

Steven T Peper

doi:10.1007/978-1-0716-2760-0_15

Molecular Surveillance of West Nile Virus in Mosquitoes and Sentinel Chickens

Methods Mol Biol. 2023:2585:157-169. doi: 10.1007/978-1-0716-2760-0_15.

Author

Steven T Peper¹

Affiliation

¹ Anastasia Mosquito Control District, St. Augustine, FL, USA. speper@amcdfl.org.

PMID: 36331773
DOI: 10.1007/978-1-0716-2760-0_15

Abstract

Arboviral surveillance is a critical step in any effective mosquito control program. Surveillance aids in the early detection of pathogen transmission as well as establishes a baseline of transmission activity. Two of the most practical forms of arboviral surveillance is through the use of testing mosquito pools for the presence of pathogen and screening sentinel chickens for pathogen exposure. This chapter describes the process for each of these methods for West Nile virus.

Keywords: ELISA; Molecular techniques; Mosquitoes; PCR; Sentinel chickens; West Nile virus.

MeSH terms

Animals
Arboviruses*
Chickens
Culicidae*
West Nile Fever* / epidemiology
West Nile Fever* / veterinary
West Nile virus* / genetics