Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection

Farzana Nazneen; Fengwei Bai

doi:10.1007/978-1-0716-2760-0_4

Isolate and Culture Mouse Primary Neurons for West Nile Virus Infection

Methods Mol Biol. 2023:2585:23-31. doi: 10.1007/978-1-0716-2760-0_4.

Authors

Farzana Nazneen¹, Fengwei Bai²

Affiliations

¹ Department of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences, The University of Southern Mississippi, Hattiesburg, MS, USA.
² Department of Cell and Molecular Biology, Center for Molecular and Cellular Biosciences, The University of Southern Mississippi, Hattiesburg, MS, USA. fengwei.bai@usm.edu.

Abstract

Primary neurons are very valuable cells to study the pathogenesis of neurotropic viruses, such as West Nile virus. The mouse primary neurons can be used to assess viral infection profiles and cellular immune responses to a viral infection. However, successful isolation and culture of mouse neurons can be challenging. Here, we report a step-by-step method to prepare a primary neuron culture from adult mice.

Keywords: Culture protocol; Mouse primary neuron; Neurotrophic virus; West Nile virus.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Immunity, Cellular
Mice
Neurons / physiology
West Nile Fever*
West Nile virus*

Abstract

Publication types

MeSH terms

Grants and funding