Viral Titer Quantification of West Nile Virus by Immunostaining Plaque Assay

Na Li; Cheng-Lin Deng; Bo Zhang; Han-Qing Ye

doi:10.1007/978-1-0716-2760-0_3

Viral Titer Quantification of West Nile Virus by Immunostaining Plaque Assay

Methods Mol Biol. 2023:2585:15-21. doi: 10.1007/978-1-0716-2760-0_3.

Authors

Na Li¹, Cheng-Lin Deng¹, Bo Zhang², Han-Qing Ye¹

Affiliations

¹ Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China.
² Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China. zhangbo@wh.iov.cn.

PMID: 36331761
DOI: 10.1007/978-1-0716-2760-0_3

Abstract

Immunostained plaque assay based on the specific antibody binding to viral antigen enables the detection and titration of virus infectivity, especially for viruses that could not form plaques using the classical crystal violet or neutral red staining methods. Here we describe the application of this method to quantify viral titers of wild-type West Nile virus (WNV-WT) and replication-defective WNV-ΔNS1 virus.

Keywords: Immunostained plaque assay; Titer quantification; Viral antigen; WNV-ΔNS1 virus; West Nile virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral
Humans
Serologic Tests
Viral Load
Viral Plaque Assay
Virus Replication
West Nile Fever*
West Nile virus*

Substances

Antibodies, Viral