Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction

Hyungsup Kim; Hyesu Kim; Luan Thien Nguyen; Taewoong Ha; Sujin Lim; Kyungmin Kim; Soon Ho Kim; Kyungreem Han; Seung Jae Hyeon; Hoon Ryu; Yong Soo Park; Sang Hyun Kim; In-Beom Kim; Gyu-Sang Hong; Seung Eun Lee; Yunsook Choi; Lawrence B Cohen; Uhtaek Oh

doi:10.1016/j.pneurobio.2022.102369

Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction

Prog Neurobiol. 2022 Dec:219:102369. doi: 10.1016/j.pneurobio.2022.102369. Epub 2022 Oct 28.

Authors

Hyungsup Kim¹, Hyesu Kim², Luan Thien Nguyen³, Taewoong Ha¹, Sujin Lim³, Kyungmin Kim¹, Soon Ho Kim¹, Kyungreem Han¹, Seung Jae Hyeon¹, Hoon Ryu¹, Yong Soo Park⁴, Sang Hyun Kim⁴, In-Beom Kim⁴, Gyu-Sang Hong¹, Seung Eun Lee¹, Yunsook Choi⁵, Lawrence B Cohen⁵, Uhtaek Oh⁶

Affiliations

¹ Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
² Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea.
³ Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
⁴ Department of Anatomy, Catholic Institute for Applied Anatomy, College of Medicine, Catholic University of Korea, Seoul 06591, Republic of Korea.
⁵ Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA.
⁶ Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: utoh@kist.re.kr.

PMID: 36330924
DOI: 10.1016/j.pneurobio.2022.102369

Abstract

Sensing smells of foods, prey, or predators determines animal survival. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca²⁺ play a significant role in transducing odorant inputs to electrical activity. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Ano9-deficient mice had reduced olfactory behavioral sensitivity, electro-olfactogram signals, and neural activity in the olfactory bulb. In line with the difference in olfaction between birds and other vertebrates, chick ANO9 failed to respond to odorants, whereas chick CNGA2, a major transduction channel, showed greater responses to cAMP. Thus, we concluded that the signal amplification by ANO9 is important for mammalian olfactory transduction.

Keywords: Amplification; Anoctamin 9; Cation channel; Olfaction; TMEM16J; cAMP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anoctamins / metabolism
Mammals / metabolism
Mice
Odorants
Olfactory Bulb / metabolism
Olfactory Receptor Neurons* / metabolism
Smell* / physiology

Substances

Anoctamins
Ano9 protein, mouse