Purple acid phosphatases in coffee-genome-wide identification and the transcriptional profile of selected members in roots upon Pi starvation

Diego Júnior Martins Vilela; Renan Terassi Pinto; Thiago Bérgamo Cardoso; Luciano Vilela Paiva; Marco Aurélio Carbone Carneiro; Gladyston Rodrigues Carvalho; Jessé Valentim Dos Santos

doi:10.1007/s13205-022-03399-6

Purple acid phosphatases in coffee-genome-wide identification and the transcriptional profile of selected members in roots upon Pi starvation

3 Biotech. 2022 Dec;12(12):335. doi: 10.1007/s13205-022-03399-6. Epub 2022 Nov 1.

Authors

Diego Júnior Martins Vilela¹, Renan Terassi Pinto², Thiago Bérgamo Cardoso³, Luciano Vilela Paiva², Marco Aurélio Carbone Carneiro⁴, Gladyston Rodrigues Carvalho⁵, Jessé Valentim Dos Santos⁴

Affiliations

¹ Agricultural Research Company of Minas Gerais (Epamig), Epamig Oeste, Patrocínio, MG Brazil.
² Laboratory of Molecular Biology, Department of Chemistry, Federal University of Lavras, Lavras, MG Brazil.
³ Laboratory of Genetics and Biotechnology, Embrapa Agroenergy, Brasilia, DF Brazil.
⁴ Department of Soil Science, Federal University of Lavras, Lavras, MG Brazil.
⁵ Agricultural Research Company of Minas Gerais (Epamig), Epamig Sul, Lavras, MG Brazil.

Abstract

Phosphorus (P) availability is determinant for crop productivity and, despite the sufficient amount of this nutrient in arable land, most of it remains insoluble, leading to the need of high fertilizer input. To cope with P scarcity forecasts and also for cropping costs alleviation, genotypes better adapted to promote P solubilization and absorption are required, especially for perennial crops. Coffee is one of these important perennial crops cultivated in soils with low P availability, and thus we aimed to study adaptive strategies to coffee genotypes in acquire phosphorus. In this study, we focused on rhizosphere phosphatase activity, a major characteristic related to P solubilization from organic pools. To explore the genetic basis of this characteristic, we firstly identified 29 Purple Acid Phosphatases (PAP) genes in C. canephora genome and selected five candidates with higher potential to encode secreted PAPs. We found that C. arabica genotypes have diverse profiles of rhizosphere phosphatase activity, as well as microbial biomass carbon, which we measured to explore the impact of the plant on microbiota and also to discriminate the phosphatase activity origin (plant or microorganism-derived). We selected two C. arabica cultivars with contrasting phosphatase activity and found that one PAP gene has a correlated gene expression profile with this characteristic. This work explores coffee adaptative responses to P starvation conditions, and the information provided can further contribute to breeding programs aiming better adapted genotypes for sustainable agriculture in face of current challenges.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-022-03399-6.

Keywords: Coffea arabica; Coffea canephora; Gene expression; PAP; Phosphorus.

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