Multigene manipulation of photosynthetic carbon metabolism enhances the photosynthetic capacity and biomass yield of cucumber under low-CO2 environment

Zhi-Feng Chen; Tian-Hong Wang; Chao-Yang Feng; Hai-Feng Guo; Xiao-Xi Guan; Tian-Li Zhang; Wen-Zhao Li; Guo-Ming Xing; Sheng Sun; Guo-Fei Tan

doi:10.3389/fpls.2022.1005261

Multigene manipulation of photosynthetic carbon metabolism enhances the photosynthetic capacity and biomass yield of cucumber under low-CO₂ environment

Front Plant Sci. 2022 Oct 18:13:1005261. doi: 10.3389/fpls.2022.1005261. eCollection 2022.

Authors

Affiliations

¹ College of Biology and Agricultural Technology, Zunyi Normal College, Zunyi, China.
² Fruit and Vegetable Research Institute, Academy of Agricultural Sciences, Zunyi, China.
³ College of Horticulture, Shanxi Agricultural University, Jinzhong, China.
⁴ Institute of Horticulture, Guizhou Academy of Agricultural Sciences, Guiyang, China.

Abstract

Solar greenhouses are important in the vegetable production and widely used for the counter-season production in the world. However, the CO₂ consumed by crops for photosynthesis after sunrise is not supplemented and becomes chronically deficient due to the airtight structure of solar greenhouses. Vegetable crops cannot effectively utilize light resources under low-CO₂ environment, and this incapability results in reduced photosynthetic efficiency and crop yield. We used cucumber as a model plant and generated several sets of transgenic cucumber plants overexpressing individual genes, including β-carbonic anhydrase 1 (CsβCA1), β-carbonic anhydrase 4 (CsβCA4), and sedoheptulose-1,7-bisphosphatase (CsSBP); fructose-1,6-bisphosphate aldolase (CsFBA), and CsβCA1 co-expressing plants; CsβCA4, CsSBP, and CsFBA co-expressing plants (14SF). The results showed that the overexpression of CsβCA1, CsβCA4, and 14SF exhibited higher photosynthetic and biomass yield in transgenic cucumber plants under low-CO₂ environment. Further enhancements in photosynthesis and biomass yield were observed in 14SF transgenic plants under low-CO₂ environment. The net photosynthesis biomass yield and photosynthetic rate increased by 49% and 79% compared with those of the WT. However, the transgenic cucumbers of overexpressing CsFBA and CsSBP showed insignificant differences in photosynthesis and biomass yield compared with the WT under low-CO₂.environment. Photosynthesis, fluorescence parameters, and enzymatic measurements indicated that CsβCA1, CsβCA4, CsSBP, and CsFBA had cumulative effects in photosynthetic carbon assimilation under low-CO₂ environment. Co-expression of this four genes (CsβCA1, CsβCA4, CsSBP, and CsFBA) can increase the carboxylation activity of RuBisCO and promote the regeneration of RuBP. As a result, the 14SF transgenic plants showed a higher net photosynthetic rate and biomass yield even under low-CO₂environment.These findings demonstrate the possibility of cultivating crops with high photosynthetic efficiency by manipulating genes involved in the photosynthetic carbon assimilation metabolic pathway.

Keywords: carbon metabolism; cucumber; greenhouse; highphotosynthetic efficiency; low-CO2; multigene manipulation.