Maternal organic selenium supplementation during gestation enhances muscle fiber area and muscle fiber maturation of offspring in porcine model

Yan Lin; Hui Yan; Lei Cao; Daolin Mou; Dajiang Ding; Binting Qin; Lianqiang Che; Zhengfeng Fang; Shengyu Xu; Yong Zhuo; Jian Li; Jianping Wang; Chao Huang; Yuanfeng Zou; Lixia Li; De Wu; Bin Feng

doi:10.1186/s40104-022-00773-5

Maternal organic selenium supplementation during gestation enhances muscle fiber area and muscle fiber maturation of offspring in porcine model

J Anim Sci Biotechnol. 2022 Nov 4;13(1):121. doi: 10.1186/s40104-022-00773-5.

Authors

Yan Lin^#^{1

2}, Hui Yan^#^{1

2}, Lei Cao^{1

2}, Daolin Mou^{1

2}, Dajiang Ding^{1

2}, Binting Qin^{1

2}, Lianqiang Che^{1

2}, Zhengfeng Fang^{1

2}, Shengyu Xu^{1

2}, Yong Zhuo^{1

2}, Jian Li^{1

2}, Jianping Wang^{1

2}, Chao Huang³, Yuanfeng Zou³, Lixia Li³, De Wu^{4

5}, Bin Feng^{6

7}

Affiliations

¹ Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China.
² Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, China.
³ College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
⁴ Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China. wude@sicau.edu.cn.
⁵ Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, China. wude@sicau.edu.cn.
⁶ Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China. fengbin@sicau.edu.cn.
⁷ Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, China. fengbin@sicau.edu.cn.

^# Contributed equally.

Abstract

Background: Organic selenium supplementation during gestation improves the antioxidant status and reproductive performance of sows and increases the antioxidative capacity of the intestines of their offspring. This study was conducted to investigate the effect of maternal basel diet (control) supplemented with an organic Se, 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), or inorganic sodium selenite (Na₂SeO₃) during gestation on the antioxidant status and development of muscle in newborn and weaned piglets. Newborn piglets before colostrum intake and weaned piglets were selected for longissimus dorsi (LD) muscle collection and analysis.

Results: The results showed that maternal HMSeBA supplementation increased the muscle area and content of Se in the LD muscle of newborn piglets, improved gene expression of selenoproteins, and decreased oxidative status in the LD muscle of both newborn and weaned piglets compared with the control. The expression of muscle development-related genes of newborn piglets in the HMSeBA group was lower than in the control group, whereas the expression of MRF4 in weaned piglets was higher in the HMSeBA group than in the control and Na₂SeO₃ groups. In addition, HMSeBA supplementation decreased the mRNA expressions of myosin heavy chains (MyHC) IIx and MyHC IIb and the percentage of MyHC IIb; increased the expression of PGC-1α in the LD muscle of newborn piglets; increased the gene expression of MyHC IIa; and decreased the protein expression of slow MyHC and the activity of malate dehydrogenase in the LD muscle of weaned piglets compared with the control group.

Conclusions: Maternal HMSeBA supplementation during gestation can improve the antioxidative capacity of the muscle of their offspring and promote the maturity of muscle fibres in weaned offspring.

Keywords: Antioxidation; HMSeBA; Muscle; Offspring; Sows.

Abstract

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