Quantitative real time PCR detection of Saboya virus: A flavivirus member of yellow fever genetic group

Idrissa Dieng; Mignane Ndiaye; Moussa Dia; Moufid Mhamadi; Cheikh Talibouya Toure; Aboubacry Gaye; Cheikh Tidiane Diagne; Ahmed El Wahed; Manfred Weidmann; Ousmane Faye; Amadou Alpha Sall; Oumar Faye

doi:10.1016/j.jviromet.2022.114638

Quantitative real time PCR detection of Saboya virus: A flavivirus member of yellow fever genetic group

J Virol Methods. 2023 Jan:311:114638. doi: 10.1016/j.jviromet.2022.114638. Epub 2022 Nov 1.

Affiliations

¹ Arboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar, Senegal. Electronic address: Idrissa.DIENG@pasteur.sn.
² Arboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar, Senegal.
³ Epidemiology, Data Sciences and Clinical Research, Senegal.
⁴ Arboviruses and Haemorrhagic Fever Viruses Unit, Virology Department, Institut Pasteur de Dakar, Dakar, Senegal; DIATROPIX, Institut Pasteur de Dakar, Dakar, Senegal.
⁵ Institute of Animal Hygiene and Veterinary Public Health, Leipzig University, Leipzig, Germany.
⁶ Institute for Microbiology and Virology, Brandenburg Medical School Theodor Fontane, Germany.

PMID: 36328081
DOI: 10.1016/j.jviromet.2022.114638

Abstract

The genus Flavivirus in the Flaviridae contains arthropod born viruses associated with high public health burdens like Zika, Dengue or Yellow fever. Saboya virus (SABV) is an understudied flavivirus grouping in the same genetic sub-group as Yellow Fever Virus (YFV) together with Sepik virus (SEPV) and Wesselbron virus (WSLV). Flavivirus infections are characterized by non-specific clinical presentations resulting in a high risk of misdiagnosis. SABV virus has been shown to circulate in the Sahelian zone and in central Africa. To study this virus we a qRT-PCR system based on TaqMan chemistry was developed to allow rapid and specific detection of SABV. The SABV assay was evaluated on available SABV isolates and others flaviviruses (DENV, ZIKV, YFV, WNV, KEDV). The system reliably detected all used SABV strains without cross amplification of other flaviviruses. In term of sensitivity the SABV assay detect up to 40.25 copies of SABV standard DNA molecule per ul. This system can be easily added to the available panel of arboviruses detection assays as a reliable tool to study virus prevalence in human, vertebrate and insect-vector samples.

Keywords: Arbovirus; Flavivirus; Saboya virus; Yellow fever genetic group; qRT-PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dengue Virus*
Flavivirus* / genetics
Humans
Real-Time Polymerase Chain Reaction
Yellow Fever* / diagnosis
Yellow fever virus / genetics
Zika Virus Infection*
Zika Virus*