Colorimetric aptasensor for fumonisin B1 detection based on the DNA tetrahedra-functionalized magnetic beads and DNA hydrogel-coated bimetallic MOFzyme

Yuhan Sun; Shuo Qi; Xiaoze Dong; Mingwei Qin; Ning Ding; Yin Zhang; Zhouping Wang

doi:10.1016/j.jhazmat.2022.130252

Colorimetric aptasensor for fumonisin B₁ detection based on the DNA tetrahedra-functionalized magnetic beads and DNA hydrogel-coated bimetallic MOFzyme

J Hazard Mater. 2023 Feb 5;443(Pt B):130252. doi: 10.1016/j.jhazmat.2022.130252. Epub 2022 Oct 25.

Authors

Yuhan Sun¹, Shuo Qi¹, Xiaoze Dong¹, Mingwei Qin¹, Ning Ding¹, Yin Zhang², Zhouping Wang³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China. Electronic address: wangzp@jiangnan.edu.cn.

PMID: 36327850
DOI: 10.1016/j.jhazmat.2022.130252

Abstract

The toxicity and incidence of fumonisin B₁ (FB₁) pose a major challenge to public health and the environment, prompting the development of alternative quantitative strategies for FB₁. Herein, a colorimetric aptasensor was constructed based on DNA tetrahedra-functionalized magnetic beads (MBs) and DNA hydrogel-coated Mn-Zr bimetallic metal-organic frameworks-based nanozyme (MOFzyme). Initially, MBs functionalized by DNA tetrahedra demonstrated excellent capturing capability for FB₁. Along with the capture of FB₁, catalyst DNA (C) was released into the supernatant. Aided by fuel DNA (F), C can trigger continuous cleavage of the main chains and cross-linking points of the DNA hydrogel through an entropy-driven DNA circuit integrated into the hydrogel coating. Subsequently, the bimetallic MOFzyme encapsulated inside the DNA hydrogel was exposed and exerted its superb peroxidase-like activity, producing a colorimetric signal whose intensity was positively dependent on the amount of FB₁. The developed aptasensor exhibited good linearity in the range of 5 × 10^-4 to 50 ng mL^-1 with a limit of detection (LOD) of 0.38 pg mL^-1, and reasonable specificity in different matrices. Furthermore, the aptasensor was successfully applied to quantify FB₁ in actual samples with recoveries fell within 92.25 %- 108.00 %, showing its great potential in environmental monitoring and food safety.

Keywords: DNA hydrogel; DNA tetrahedra; Fumonisin B(1); Nanozyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
Colorimetry*
DNA
Gold
Hydrogels
Limit of Detection
Magnetic Phenomena

Substances

fumonisin B1
Gold
Hydrogels
DNA