A novel highly selective FRET sensor for Fe(III) and DFT mechanistic evaluation

Ketsarin Chantarasunthon; Malinee Promkatkaew; Patthreera Waranwongcharoen; Anek Sueksachat; Nitchanan Prasop; Thanaporn Norasi; Narisa Sonsiri; Sirirat Sansern; Sinchai Chomngam; Kanokorn Wechakorn; Chanat Thana; Wissawat Sakulsaknimitr; Palangpon Kongsaeree; Pailin Srisuratsiri

doi:10.1016/j.saa.2022.122031

A novel highly selective FRET sensor for Fe(III) and DFT mechanistic evaluation

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Feb 5:286:122031. doi: 10.1016/j.saa.2022.122031. Epub 2022 Oct 22.

Authors

Affiliations

¹ Department of Basic Science and Physical Education, Faculty of Science at Sriracha, Kasetsart University Sriracha Campus, Chonburi 20230, Thailand.
² Department of Chemistry and Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
³ Department of Chemistry, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathumthani 12110, Thailand.
⁴ Department of Basic Science and Physical Education, Faculty of Science at Sriracha, Kasetsart University Sriracha Campus, Chonburi 20230, Thailand. Electronic address: srisuratsiri.p@ku.th.

PMID: 36323091
DOI: 10.1016/j.saa.2022.122031

Abstract

A novel FRET-based sensor has been designed and developed through the conjugation of naphthyl and rhodamine via propylamine spacer, Naph-Rh. The naphthyl moiety serves as a FRET donor due to its emission spectrum overlapping with the rhodamine B absorption band. Naph-Rh exhibited a selectivity for sensing Fe³⁺ over other metal ions with a visual color change and fluorescent enhancement. The ratio of the Naph-Rh and Fe³⁺ was determined to be 1:1 based on Job's plot analysis with a detection limit of 83 nM. The probe exhibited a linear response to Fe³⁺ in the range of 0-120 μM. Furthermore, the density functional theory (DFT) calculations of Naph-Rh were carried out to rationalize the design and portray the plausible Fe³⁺ sensing mechanism.

Keywords: FRET sensor; Fluorescence sensor; Iron detection; Naphthyl; Rhodamine.

MeSH terms

Fluorescence Resonance Energy Transfer*
Fluorescent Dyes*
Ions
Iron / analysis
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Iron
Ions