Deciphering Alternaria metabolic responses in microbial confrontation via an integrated mass spectrometric targeted and non-targeted strategy

Ye Tian; Mohamed F Abdallah; Marthe De Boevre; Kris Audenaert; Cheng Wang; Sarah De Saeger; Aibo Wu

doi:10.1016/j.foodchem.2022.134694

Deciphering Alternaria metabolic responses in microbial confrontation via an integrated mass spectrometric targeted and non-targeted strategy

Food Chem. 2023 Mar 15;404(Pt B):134694. doi: 10.1016/j.foodchem.2022.134694. Epub 2022 Oct 20.

Authors

Ye Tian¹, Mohamed F Abdallah², Marthe De Boevre³, Kris Audenaert⁴, Cheng Wang⁵, Sarah De Saeger³, Aibo Wu⁶

Affiliations

¹ SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
² Centre of Excellence in Mycotoxicology and Public Health, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Assiut University, Assiut 71515, Egypt.
³ Centre of Excellence in Mycotoxicology and Public Health, Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
⁴ Laboratory of Applied Mycology and Phenomics, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium.
⁵ Institute of Quality Standards and Testing Technology for Agro-Products, Key Laboratory of Agro-Products Quality and Safety of Xinjiang, Laboratory of Quality and Safety Risk Assessment for Agro-Products (Urumqi), Ministry of Agriculture, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang 830091, China.
⁶ SIBS-UGENT-SJTU Joint Laboratory of Mycotoxin Research, CAS Key Laboratory of Nutrition, Metabolism and Food Safety, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address: abwu@sinh.ac.cn.

PMID: 36323034
DOI: 10.1016/j.foodchem.2022.134694

Abstract

Microbial confrontation is ubiquitously present in nature, such as mycoparasitism, competition and antibiosis between biocontrol agents and microbial pathogens. However, the internal metabolic responses of fungal pathogens under microbial interaction scenario have been scarcely investigated. In this study, we set up an integrated mycotoxin analysis and non-targeted metabolomics workflow to decipher metabolic changes of Alternaria pathogens when confronted with selected Trichoderma strains, as well as mycotoxin metabolization in the Trichoderma spp. Results demonstrated that Trichoderma spp. significantly influenced mycotoxin production and whole metabolome of Alternaria pathogens when in cocultivation, and one Trichoderma strain could metabolize alternariol into its hydroxylated form. These differential metabolites revealed fungal physiological alternations in various confrontation conditions. In all, a MS-based strategy was proposed to investigate microbial metabolic profiles under fungal/fungal and fungal/mycotoxin cocultivation, and this generic methodology would be significant for understanding the occurrence and change of food contaminants in microbial interactions.

Keywords: Alternaria; Metabolic profiling; Microbial confrontation; Mycotoxin; Trichoderma.

MeSH terms

Alternaria / metabolism
Antibiosis
Mycotoxins* / metabolism
Plant Diseases / microbiology
Trichoderma* / genetics
Trichoderma* / metabolism

Substances

Mycotoxins