Characterization of high Arabinoxylan oat lines identified from a mutagenized oat population

José Alfredo Zambrano; Aishwarya Thyagarajan; Roya R R Sardari; Olof Olsson

doi:10.1016/j.foodchem.2022.134687

Characterization of high Arabinoxylan oat lines identified from a mutagenized oat population

Food Chem. 2023 Mar 15;404(Pt B):134687. doi: 10.1016/j.foodchem.2022.134687. Epub 2022 Oct 19.

Authors

José Alfredo Zambrano¹, Aishwarya Thyagarajan², Roya R R Sardari³, Olof Olsson⁴

Affiliations

¹ Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, SE 22100 Lund, Sweden.
² CropTailorAB, c/o Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, SE 22100 Lund, Sweden.
³ Department of Biotechnology, Lund University, Box 124, SE 22100 Lund, Sweden.
⁴ Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, SE 22100 Lund, Sweden; CropTailorAB, c/o Division of Pure and Applied Biochemistry, Department of Chemistry, Lund University, Box 124, SE 22100 Lund, Sweden. Electronic address: olof.olsson@tbiokem.lth.se.

PMID: 36323030
DOI: 10.1016/j.foodchem.2022.134687

Abstract

Oat (Avena sativa) is a nutritionally important cereal crop that is rich in health-promoting dietary fibers, favorable proteins and polar lipids. In this work, ca. 500 random lines of a mutagenized oat population of high genetic variation were screened for arabinoxylan (AX) content. This identified lines with up to 60% higher AX levels in flour from whole seed and up to 100% higher in flour from dehulled seeds, as compared to the original Belinda variety. In addition, the cellular localization of AX was determined in cross-sections of dehulled seeds from three high and one low AX line using a xylan-specific antibody. This revealed variations in the amount and localization of AX between high and low AX lines. The high AX lines will now serve as a starting point in the development of oat varieties with superior health-promoting and rheological properties.

Keywords: Arabinoxylan (AX); Fibers; High oat AX lines; Immunolocalization; Mutagenesis; Oat.

MeSH terms

Avena* / genetics
Avena* / metabolism
Edible Grain / metabolism
Flour / analysis
Xylans* / metabolism

Substances

arabinoxylan
Xylans