Enhancement of biocontrol efficacy of Kluyveromyces marxianus induced by N-acetylglucosamine against Penicillium expansum

Yalan Wu; Yufang Gao; Xiaodong Zheng; Ting Yu; Fujie Yan

doi:10.1016/j.foodchem.2022.134658

Enhancement of biocontrol efficacy of Kluyveromyces marxianus induced by N-acetylglucosamine against Penicillium expansum

Food Chem. 2023 Mar 15;404(Pt B):134658. doi: 10.1016/j.foodchem.2022.134658. Epub 2022 Oct 19.

Authors

Yalan Wu¹, Yufang Gao¹, Xiaodong Zheng¹, Ting Yu², Fujie Yan³

Affiliations

¹ National Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
² National Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: yuting@zju.edu.cn.
³ National Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: fjyan@zju.edu.cn.

PMID: 36323019
DOI: 10.1016/j.foodchem.2022.134658

Abstract

This research aimed to investigate the effect of N-acetylglucosamine (GlcNAc) on the biocontrol activity of Kluyveromyces marxianus and involved possible mechanisms. The results indicated that 0.5% GlcNAc significantly improved the antagonistic efficacy of K. marxianus against Penicillium expansum. GlcNAc supplementation led to increases of biocontrol-related enzymes and stress-resistant substances in K. marxianus including chitinase, β-1,3-glucanase, trehalose and proline, being beneficial for yeasts vigor maintenance under adverse circumstances. The antioxidative-related enzyme activities in K. marxianus, such as superoxide dismutase (SOD), catalase (CAT) and others, were increased by GlcNAc along with more quickly excess reactive oxygen species (ROS) scavenging. GlcNAc resulted in an enhancement of proliferative capacity in K. marxianus according to the transcriptomic analysis. Consequently, GlcNAc contributed to improvements of K. marxianus in the environmental adaptability and stress resistance. Our study demonstrated that both GlcNAc and K. marxianus could be regarded as potential candidates for postharvest technology application.

Keywords: Biocontrol efficacy; Kluyveromyces marxianus; N-acetylglucosamine; Penicillium expansum; Transcriptome.

MeSH terms

Acetylglucosamine
Kluyveromyces* / genetics
Penicillium*

Substances

Acetylglucosamine

Supplementary concepts

Kluyveromyces marxianus
Penicillium expansum