Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea

BMC Vet Res. 2022 Nov 1;18(1):382. doi: 10.1186/s12917-022-03474-3.

Abstract

Host genotype is important for enterotoxigenic E. coli (ETEC) susceptibility. We conducted two trials to evaluate the effect of CHCF1 genotype on incidence of ETEC diarrhea. In trial 1 (n = 15 pigs), pigs were inoculated with 108 CFU or 1010 CFU doses of an ETEC F4ac strain. In trial 2 (n = 33 pigs), pigs were inoculated with ETEC F4ab or F4ac. Across trials, all inoculated pigs that developed ETEC diarrhea were CHCF1 heterozygous susceptible (6/6). No inoculated CHCF1 homozygous resistant pigs developed ETEC diarrhea (0/26). Susceptibility towards ETEC F4ac/ab infection might correspond with CHCF1 genotype.

Keywords: Diarrhea; Enterotoxigenic Escherichia coli; Experimental infection; F4 fimbriae; Genetic marker; Inoculum dose; Piglet; Post-weaning.

MeSH terms

  • Animals
  • Diarrhea / veterinary
  • Enterotoxigenic Escherichia coli*
  • Escherichia coli Infections* / veterinary
  • Genotype
  • Pilot Projects
  • Swine
  • Swine Diseases* / genetics
  • Weaning