The fruit peel of a color mutant jujube cultivar, 'Sanbianhong' (SBF), was investigated using an ultra-high performance liquid chromatography quadrupole Orbitrap mass spectrometry (UHPLC-Q-Orbitrap MS) at five ripening stages (S1, Young fruit stage; S2, swelling stage; S3, white-mature stage; S4, pre-mature stage and S5, mature stage). Lutein, β-carotene, chlorophyll a, chlorophyll b, and 13 anthocyanins were identified. Chlorophyll a and cyanidin 3-O-galactoside were considered key color metabolites in S1 with the content of 1.083 mg/g of fresh weight (FW) and 4.585 mg/g of FW, respectively. Delphinidin (0.488 mg/g FW) and cyanidin (6.259 mg/g FW) were identified as the key pigments in S3. Delphinidin 3-O-glucoside (0.256 mg/g FW) was identified as the key anthocyanin in maturity S5. Herein, the identification and quantitation of pigment-related metabolites of SBF were studied for the first time, and the results provide a theoretical basis for understanding the pigment changes of jujube fruit during ripening.
Keywords: AGC, automatic gain control; BHT, butylated hydroxytoluene; FC, fold change; HCD, higher-energy collision dissociation; HESI, heat electrospray ionization; Jujube peel; KEGG, Kyoto Encyclopedia of Genes and Genomes; LOD, limit of detection; LOQ, limit of quantification; Metabolome; NCE, normalized collision energy; OPLS-DA, orthogonal projections to latent structures-discriminant analysis; PCA, principle component analysis; PRM, parallel-reaction monitor; Pigmentation; Quantification standards; RT, retention time; SBF, ‘Sanbianhong’; SRM, selected reaction monitoring; UHPLC-Q-Orbitrap MS, ultra-high performance liquid chromatography quadrupole Orbitrap mass spectrometry; VIP, variable importance in projection.
© 2022 The Authors.