LIST: A Newly Developed Laser-assisted Cell Bioprinting Technology

Katiane Roversi; Hamid Ebrahimi Orimi; Mahyar Erfanian; Sebastien Talbot; Christos Boutopoulos

doi:10.21769/BioProtoc.4527

LIST: A Newly Developed Laser-assisted Cell Bioprinting Technology

Bio Protoc. 2022 Oct 5;12(19):e4527. doi: 10.21769/BioProtoc.4527.

Authors

Katiane Roversi^{1

2

3}, Hamid Ebrahimi Orimi^{2

4}, Mahyar Erfanian^{2

5}, Sebastien Talbot¹, Christos Boutopoulos^{2

3

5}

Affiliations

¹ Département de Pharmacologie et Physiologie, Université de Montréal, Montréal, QC, Canada.
² Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC, Canada.
³ Département d'Ophtalmologie, Université de Montréal, Montréal, QC, Canada.
⁴ Department of Mechanical, Industrial and Aerospace Engineering, Concordia University, Montréal, QC, Canada.
⁵ Institut de Génie Biomédical, Université de Montréal, Montréal, QC, Canada.

Abstract

Cell bioprinting technologies aim to fabricate tissue-like constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. We recently introduced a laser-assisted drop-on-demand bioprinting technology termed Laser Induced Side Transfer (LIST). This technology can print delicate cell types, including primary neurons. This bioprinting protocol includes the following key steps: cell harvesting, bio-ink preparation, laser setup priming, printing, and post-printing analysis. This protocol includes a detailed description of the laser setup, which is a rather unusual setup for a biology lab. This should allow easy reproduction by readers with basic knowledge of optics. Although we have focused on neuron bioprinting, interested readers will be able to adapt the protocol to bioprint virtually any cell type.

Keywords: 3D bio-printing; Drop-on-demand; Laser-assisted bioprinting; Laser-induced side transfer; Nociceptor neurons; Tissue engineering.