Protein from Hylocereus polyrhizus protects MRC-5 cells against hydrogen peroxide (H2O2)-induced damage

Haomiao Ding; Yuzhe Zhang; Yue Zha; Sijia Zhou; Chaoqing Cao; Huajian Zhu; Feng Xu; Xiuqiang Zhang; Caisheng Wang

doi:10.1007/s10068-022-01163-3

Protein from Hylocereus polyrhizus protects MRC-5 cells against hydrogen peroxide (H₂O₂)-induced damage

Food Sci Biotechnol. 2022 Oct 12;31(13):1741-1751. doi: 10.1007/s10068-022-01163-3. eCollection 2022 Dec.

Authors

Haomiao Ding¹, Yuzhe Zhang¹, Yue Zha¹, Sijia Zhou¹, Chaoqing Cao¹, Huajian Zhu¹, Feng Xu², Xiuqiang Zhang², Caisheng Wang¹

Affiliations

¹ College of Biological and Environmental Sciences, Zhejiang Wanli University, 8 Qianhu South Road, Ningbo, 315100 China.
² The Affiliated Hospital of Medical School of Ningbo University, 247 Renmin Road, Ningbo, 315099 China.

Abstract

The cytoprotective and potential molecular mechanisms of Hylocereus polyrhizus protein (RFPP) were investigated on the hydrogen peroxide (H₂O₂)-triggered damage in normal human embryonic lung (MRC-5) cells. An MTT assay was conducted to assess the MRC-5 cell viability after exposure to H₂O₂ or RFPP. Cell cycle distribution and apoptosis were explored via flow cytometry. The contents of related proteins were assessed via western blot. MRC-5 cells exhibited markedly decreased cellular viability after treatment with H₂O₂; however, treatment with RFPP suppressed this decrease. Additionally, RFPP interference dampened H₂O₂-triggered intracellular apoptosis levels and increased H₂O₂-triggered intracellular S phase. In these processes, the contents of phosphorylated (p)-AKT along with p-mTOR proteins were downregulated in 120 µM H₂O₂-treated cells compared with vehicle-treated cells. Nevertheless, in MRC-5 cells inoculated with RFPP, the levels expression of these proteins were reversed. To conclude, RFPP protected MRC-5 cells from H₂O₂-triggered damage via activation of the PI3K/AKT/mTOR cascade.

Keywords: Damage; Hylocereus polyrhizus; MRC-5 cells; PI3K/AKT/ mTOR cascade; Protein.

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