RNA-seq reveals the role of miR-199a-3p in regulating inflammation of bovine mammary epithelial cells

Yu-Hang Li; Qian-Qian Ren; Jin-Peng Wang; Xing-Ping Wang; Zhuo-Ma Luoreng; Yun Ma; Da-Wei Wei

doi:10.1016/j.rvsc.2022.10.011

RNA-seq reveals the role of miR-199a-3p in regulating inflammation of bovine mammary epithelial cells

Res Vet Sci. 2022 Dec 31:153:57-60. doi: 10.1016/j.rvsc.2022.10.011. Epub 2022 Oct 18.

Authors

Yu-Hang Li¹, Qian-Qian Ren¹, Jin-Peng Wang¹, Xing-Ping Wang², Zhuo-Ma Luoreng³, Yun Ma¹, Da-Wei Wei¹

Affiliations

¹ School of Agriculture, Ningxia University, Yinchuan, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China.
² School of Agriculture, Ningxia University, Yinchuan, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China. Electronic address: wxp@nxu.edu.cn.
³ School of Agriculture, Ningxia University, Yinchuan, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China. Electronic address: luorenzhuoma@nxu.edu.cn.

PMID: 36308792
DOI: 10.1016/j.rvsc.2022.10.011

Abstract

MicroRNAs (miRNAs) are involved in the regulation of a variety of biological processes. However, the research on the regulatory role of bovine mammary epithelial cells (bMECs) is scarce. To date, there are no reports about the role of miR-199a-3p in bMECs. In this study, RNA sequencing (RNA-seq) technology was used to detect the transcriptomes of the miR-199a-3p overexpression and negative control (NC) groups of bMECs. Then, the screening and functional annotation of differentially expressed genes (DEGs) were conducted. The results showed that there were 140 DEGs (109 up-regulated and 31 down-regulated) in the miR-199a-3p overexpression group. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the DEGs might regulate the immune and inflammatory responses via the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway, transforming growth factor-beta (TGF-β) signaling pathway, and interleukin-17 (IL-17) signaling pathway, which revealed that miR-199a-3p might participate in regulating bMECs inflammation via affecting the expression of related genes and the above signaling pathways. This study may provide a new reference for potential therapeutic targets of cow mastitis.

Keywords: Bovine mammary epithelial cells; Dairy cow; Mastitis; RNA-seq; miR-199a-3p.