Sodium butyrate exerts antioxidant stress effects and attenuates Aβ25-35-induced cytotoxicity in PC12 cells

Boyu Yuan; Mingming Liu; Yuhong Gong; Zifan Wang; Xinxin Jin; Gaijie Xie; Mingqiang Zhu; Xue Zhang; Siyuan Luo; Qing Qu; Yufeng Zhu; Meng Wang; Yingli Jin; Bai Li; Wei Wang

doi:10.1016/j.abb.2022.109448

Sodium butyrate exerts antioxidant stress effects and attenuates Aβ_25-35-induced cytotoxicity in PC12 cells

Arch Biochem Biophys. 2022 Nov 30:731:109448. doi: 10.1016/j.abb.2022.109448. Epub 2022 Oct 25.

Authors

Boyu Yuan¹, Mingming Liu², Yuhong Gong³, Zifan Wang², Xinxin Jin², Gaijie Xie⁴, Mingqiang Zhu⁴, Xue Zhang⁴, Siyuan Luo⁴, Qing Qu⁴, Yufeng Zhu³, Meng Wang⁵, Yingli Jin⁶, Bai Li⁷, Wei Wang⁸

Affiliations

¹ Innovative Institute of Animal Healthy Breeding, College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China; Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, 130021, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
² Innovative Institute of Animal Healthy Breeding, College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China.
³ Laboratory Animal Center of Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.
⁴ Innovative Institute of Animal Healthy Breeding, College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China.
⁵ Bureau of Agriculture and Rural Affairs of Conghua District, Guangzhou, 510900, China.
⁶ Department of Pharmacology, College of Basic Medical Science, Jilin University, Changchun, 130021, China. Electronic address: jinyingli@jlu.edu.cn.
⁷ First Clinical Hospital of Jilin University, Changchun, 130021, China. Electronic address: li_bai@jlu.edu.cn.
⁸ Innovative Institute of Animal Healthy Breeding, College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China. Electronic address: wang_wei99@jlu.edu.cn.

PMID: 36306919
DOI: 10.1016/j.abb.2022.109448

Abstract

Alzheimer's disease (AD), a common neurodegenerative disease, is characterised by the deposition of amyloid-β (Aβ) plaques and neurofibrillary tangles. An increasing number of studies have demonstrated that Aβ causes neuronal damage and mitochondrial dysfunction. Herein, we evaluated the neuroprotective effect of sodium butyrate (NaB) against Aβ induced neurotoxicity in PC12 cells. The results revealed that 3 mM of NaB promoted the expression of angiotensin-converting enzyme and brain-derived neurotrophic factor, which exert a neuroprotective effect by activating G protein-coupled receptors. Moreover, NaB could significantly improve mitochondrial dysfunction caused by Aβ. In conclusion, NaB protected PC12 cells from Aβ-induced cell damage, highlighting the potential of NaB in AD treatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease* / drug therapy
Alzheimer Disease* / metabolism
Amyloid beta-Peptides / metabolism
Amyloid beta-Peptides / toxicity
Animals
Antioxidants / metabolism
Apoptosis
Butyric Acid / pharmacology
Cell Survival
Membrane Potential, Mitochondrial
Neurodegenerative Diseases*
Neuroprotective Agents* / pharmacology
Neuroprotective Agents* / therapeutic use
PC12 Cells
Peptide Fragments / metabolism
Peptide Fragments / toxicity
Rats

Substances

Amyloid beta-Peptides
Antioxidants
Butyric Acid
Neuroprotective Agents
Peptide Fragments