Construction of Small-Insert and Large-Insert Metagenomic Libraries

Carola Simon; Rolf Daniel

doi:10.1007/978-1-0716-2795-2_1

Construction of Small-Insert and Large-Insert Metagenomic Libraries

Methods Mol Biol. 2023:2555:1-12. doi: 10.1007/978-1-0716-2795-2_1.

Authors

Carola Simon¹, Rolf Daniel²

Affiliations

¹ Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany.
² Institute of Microbiology and Genetics, Georg-August-University Göttingen, Göttingen, Germany. rdaniel@gwdg.de.

PMID: 36306075
DOI: 10.1007/978-1-0716-2795-2_1

Abstract

The vast majority of the Earth's biological diversity are hidden in uncultured and yet uncharacterized microbial genomes. The construction of metagenomic libraries is one cultivation-independent molecular approach to assess this unexplored genetic reservoir. High numbers of novel biocatalysts have been identified by function-based or sequence-based screening of metagenomic libraries derived from various environments. Here, we describe detailed protocols for the construction of metagenomic small-insert and large-insert libraries in plasmids and fosmids, respectively, from environmental DNA.

Keywords: Fosmid; Large-insert library; Metagenomic DNA; Plasmid; Small-insert library; WGA; Whole-genome amplification.

MeSH terms

Biodiversity
Gene Library
Metagenome*
Metagenomics* / methods
Plasmids / genetics