Construction of homozygous diploid potato through maternal haploid induction

Jinzhe Zhang; Jian Yin; Jiayi Luo; Die Tang; Xijian Zhu; Jie Wang; Zhihong Liu; Pei Wang; Yu Zhong; Chenxu Liu; Canhui Li; Shaojiang Chen; Sanwen Huang

doi:10.1007/s42994-022-00080-7

Construction of homozygous diploid potato through maternal haploid induction

aBIOTECH. 2022 Sep 15;3(3):163-168. doi: 10.1007/s42994-022-00080-7. eCollection 2022 Sep.

Authors

Jinzhe Zhang^#¹, Jian Yin^#^{1

2

3}, Jiayi Luo^{1

2}, Die Tang², Xijian Zhu^{1

2}, Jie Wang^{1

2}, Zhihong Liu^{1

2}, Pei Wang², Yu Zhong⁴, Chenxu Liu⁴, Canhui Li³, Shaojiang Chen⁴, Sanwen Huang²

Affiliations

¹ Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Beijing, 100081 China.
² Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120 China.
³ The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming, 650500 China.
⁴ National Maize Improvement Center of China, Key Laboratory of Crop Heterosis and Utilization/Engineering Research Center for Maize Breeding, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.

^# Contributed equally.

Abstract

Reinventing the tetraploid potato into a seed-propagated, diploid, hybrid potato would significantly accelerate potato breeding. In this regard, the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes, but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines. To resolve this impediment, we developed a doubled haploid (DH) technology, based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein (StDMP) gene. Here, we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines. An exercisable protocol, involving hybridization, fluorescent marker screening, molecular and flow cytometric identification, and doubling with colchicine generates nearly 100% homozygous diploid potato lines. This dmp-triggered haploid induction (HI) system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.

Supplementary information: The online version contains supplementary material available at 10.1007/s42994-022-00080-7.

Keywords: Diploid potato; Doubled haploid technology; Haploid induction; Inbred lines; StDMP.