The Role of Recombinant Glycodelin in the Differentiation of Regulatory T-Lymphocytes

K Yu Shardina; V P Timganova; M S Bochkova; P V Khramtsov; M B Rayev; S A Zamorina

doi:10.1134/S0012496622050131

The Role of Recombinant Glycodelin in the Differentiation of Regulatory T-Lymphocytes

Dokl Biol Sci. 2022 Oct;506(1):137-140. doi: 10.1134/S0012496622050131. Epub 2022 Oct 27.

Authors

K Yu Shardina¹, V P Timganova¹, M S Bochkova^{1

2}, P V Khramtsov^{1

2}, M B Rayev^{1

2}, S A Zamorina^{3

4}

Affiliations

¹ Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Branch of the PFRC, Ural Branch of the Russian Academy of Sciences, 614081, Perm, Russia.
² Perm State National Research University, 614068, Perm, Russia.
³ Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Branch of the PFRC, Ural Branch of the Russian Academy of Sciences, 614081, Perm, Russia. zamorina.sa@gmail.com.
⁴ Perm State National Research University, 614068, Perm, Russia. zamorina.sa@gmail.com.

PMID: 36301420
DOI: 10.1134/S0012496622050131

Abstract

The effect of recombinant glycodelin (GdA) on the number of T-regulatory lymphocytes (Treg) in a culture of activated CD4⁺ lymphocytes was investigated, while simultaneously assessing the proliferative status of the cells. Recombinant GdA from E. coli and from HEK293 cells were used in the study at concentrations of 0.2; 2 and 10 µg/mL. It was found that only a low concentration (0.2 μg/mL) of recombinant GdA of bacterial origin reduced the number of proliferating CD4⁺ lymphocytes as well as the number of Treg (CD4⁺CD25^highCD127^-/low) in the experimental system in vitro.

MeSH terms

Escherichia coli
Glycodelin
HEK293 Cells
Humans
Interleukin-7 Receptor alpha Subunit* / metabolism
T-Lymphocytes, Regulatory*

Substances

Glycodelin
Interleukin-7 Receptor alpha Subunit