Immobilization of Cd2+ and Pb2+ by biomineralization of the carbonate mineralized bacterial consortium JZ1

Abstract

Microbially induced carbonate precipitation (MICP) has been proven to effectively immobilize Cd²⁺ and Pb²⁺ using a single bacterium. However, there is an urgent need for studies of Cd²⁺ and Pb²⁺ immobilized by a bacterial consortium. In this study, a stable consortium designated JZ1 was isolated from soil that was contaminated with cadmium and lead, and the dominant genus Sporosarcina (99.1%) was found to have carbonate mineralization function. The results showed that 91.52% and 99.38% of Cd²⁺ and Pb²⁺ were mineralized by the consortium JZ1 with 5 g/L CaCl₂ at an initial concentration of 5 mg/L Cd²⁺ and 150 mg/L Pb²⁺, respectively. The bioprecipitates were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Moreover, the kinetic studies indicated that the urea hydrolysis reaction fit well with the Michaelis-Menten equation, and the kinetic parameters K_m and V_max were estimated to be 38.69 mM and 58.98 mM/h, respectively. When the concentration of urea increased from 0.1 to 0.3 M, the mineralization rate increased by 1.58-fold. This study can provide a novel microbial resource for the biomineralization of Cd and Pb in soil and water environments.

Keywords: Biomineralization; Carbonate mineralized bacteria; Heavy metal; Microbially induced carbonate precipitation (MICP).