Molecular and cytogenetic dissection of stripe rust resistance gene Yr83 from rye 6R and generation of resistant germplasm in wheat breeding

Guangrong Li; Jianbo Li; Yao Zhang; Qiang Ma; Ennian Yang; Peng Zhang; Ian Dundas; Zujun Yang

doi:10.3389/fpls.2022.1035784

Molecular and cytogenetic dissection of stripe rust resistance gene Yr83 from rye 6R and generation of resistant germplasm in wheat breeding

Front Plant Sci. 2022 Oct 10:13:1035784. doi: 10.3389/fpls.2022.1035784. eCollection 2022.

Authors

Guangrong Li¹, Jianbo Li², Yao Zhang¹, Qiang Ma³, Ennian Yang⁴, Peng Zhang², Ian Dundas⁵, Zujun Yang¹

Affiliations

¹ Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
² School of Life and Environmental Sciences, Plant Breeding Institute, The University of Sydney, Cobbitty, NSW, Australia.
³ Characteristic Crops Research Institute, Chongqing Academy of Agricultural Sciences, Yongchuan, China.
⁴ Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
⁵ School of Agriculture, Food and Wine, The University of Adelaide, Waite, Glen Osmond, SA, Australia.

Abstract

Rye 6R-derived stripe rust resistance gene Yr83 in wheat background was physically mapped to fraction length (FL) 0.87-1.00 on the long arm by non-denaturing-fluorescence in situ hybridization (ND-FISH), Oligo-FISH painting and 6R-specific PCR markers.Stripe rust resistance gene Yr83 derived from chromosome 6R of rye (Secale cereale) "Merced" has displayed high resistance to both Australian and Chinese wheat stripe rust isolates. With the aim to physically map Yr83 to a more precise region, new wheat- 6R deletion and translocation lines were produced from derived progenies of the 6R(6D) substitution line. The non-denaturing fluorescence in situ hybridization (ND-FISH) patterns of 6R were established to precisely characterize the variations of 6R in different wheat backgrounds. Comparative ND-FISH analysis localized the breakpoints of 6RL chromosomes relative to Oligo-pSc200 and Oligo-pSc119.2 rich sites in deletion lines. Molecular marker and resistance analyses confirmed that Yr83 is physically located at the fraction length (FL) 0.87-1.00 of 6RL and covers the corresponding region of 806-881 Mb in the reference genome of Lo7. Oligo-FISH painting demonstrated that the region carrying Yr83 is syntenic to the distal end of long arm of homoeologous group 7 of the Triticeae genome. The developed wheat-6R lines carrying the Yr83 gene will be useful for breeding for rust resistance.

Keywords: FISH; Yr83; rye; stripe rust; wheat.