The P3N-PIPO Protein Encoded by Wheat Yellow Mosaic Virus Is a Pathogenicity Determinant and Promotes Its Pathogenicity through Interaction with NbRLK6 in Nicotiana benthamiana

Runpu Miao; Zhuangxin Ye; Stuart MacFarlane; Yanjun Li; Qianzhuo Mao; Yanzhen Tian; Zhiping Deng; Zongtao Sun; Jian Yang; Junmin Li; Fei Yan; Jianping Chen; Chulang Yu

doi:10.3390/v14102171

The P3N-PIPO Protein Encoded by Wheat Yellow Mosaic Virus Is a Pathogenicity Determinant and Promotes Its Pathogenicity through Interaction with NbRLK6 in Nicotiana benthamiana

Viruses. 2022 Sep 30;14(10):2171. doi: 10.3390/v14102171.

Authors

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
² Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.

Abstract

Similarly to other potyvirids, the bymovirus wheat yellow mosaic virus (WYMV) encodes a P3N-PIPO protein that is expressed by frameshifting occurring within the open reading frame of the P3 protein. P3N-PIPO is known to be essential for the cell-to-cell movement of several potyviruses, but this has not yet been confirmed for the WYMV. Here, we show that the WYMV P3N-PIPO protein influences disease symptom formation. Infection of Nicotiana benthamiana plants with a potato virus X (PVX)-based vector carrying the WYMV P3N-PIPO gene induced more severe disease symptoms and resulted in higher virus accumulation levels than did infection with PVX lacking the P3N-PIPO gene. N. benthamiana P3N-PIPO-interacting proteins were identified through co-immunoprecipitation (Co-IP) coupled with LC-MS/MS (mass spectrometry), and the interaction between P3N-PIPO and the N. benthamiana receptor-like kinase NbRLK6 was further verified by Co-IP and bimolecular fluorescence complementation (BiFC) of transiently-expressed proteins. Furthermore, our investigation showed that the disease symptom severity and accumulation level of PVX-P3N-PIPO were decreased in N. benthamiana plants when NbRLK6 expression was reduced by tobacco rattle virus-induced gene silencing.

Keywords: NbRLK6; P3N-PIPO; WYMV; pathogenicity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Nicotiana
Plant Diseases
Potexvirus* / genetics
Potyvirus* / genetics
Tandem Mass Spectrometry
Triticum
Viral Proteins / metabolism
Virulence

Substances

Viral Proteins

Grants and funding

This work was funded by the National Natural Science Foundation of China (Grant No.31801703). This work was sponsored by the K.C. Wong Magna Fund in Ningbo University.