Bioactive Hydroxyapatite-Magnesium Phosphate Coatings Deposited by MAPLE for Preventing Infection and Promoting Orthopedic Implants Osteointegration

Denisa Alexandra Florea; Valentina Grumezescu; Alexandra Cătălina Bîrcă; Bogdan Ștefan Vasile; Andrei Iosif; Cristina Chircov; Miruna S Stan; Alexandru Mihai Grumezescu; Ecaterina Andronescu; Mariana Carmen Chifiriuc

doi:10.3390/ma15207337

Bioactive Hydroxyapatite-Magnesium Phosphate Coatings Deposited by MAPLE for Preventing Infection and Promoting Orthopedic Implants Osteointegration

Materials (Basel). 2022 Oct 20;15(20):7337. doi: 10.3390/ma15207337.

Authors

Affiliations

¹ Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.
² National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania.
³ Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.
⁴ Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania.
⁵ Academy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, Romania.
⁶ Department of Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania.
⁷ The Romanian Academy, Calea Victoriei 25, District 1, 010071 Bucharest, Romania.

Abstract

In this study, we used the matrix-assisted pulsed laser evaporation (MAPLE) technique to obtain hydroxyapatite (Ca₁₀(PO₄)₆(OH)₂) and magnesium phosphate (Mg₃(PO₄)₂) thin coatings containing bone morphogenetic protein (BMP4) for promoting implants osteointegration and further nebulized with the antibiotic ceftriaxone (CXF) to prevent peri-implant infections. The samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), infrared microscopy (IRM) and Fourier-transform infrared spectroscopy (FT-IR). Furthermore, the antimicrobial properties were evaluated on Staphylococcus aureus biofilms and the cytocompatibility on the MC3T3-E1 cell line. The obtained results proved the potential of the obtained coatings for bone implant applications, providing a significant antimicrobial and antibiofilm effect, especially in the first 48 h, and cytocompatibility in relation to murine osteoblast cells.

Keywords: BMP4; MAPLE; Staphylococcus aureus biofilm; antibiotic-containing coatings; ceftriaxone; osteoblasts.

Grants and funding

This work was supported by the Romanian Ministry of Education and Research, CCCDI—UEFISCDI, project number PN-III-P1-1.1-TE2019-1449, and the partial support of the Core Programme 16N/2019.