Synthesis of Protein-Oligonucleotide Conjugates

Emma E Watson; Nicolas Winssinger

doi:10.3390/biom12101523

Synthesis of Protein-Oligonucleotide Conjugates

Biomolecules. 2022 Oct 20;12(10):1523. doi: 10.3390/biom12101523.

Authors

Emma E Watson¹, Nicolas Winssinger²

Affiliations

¹ Department of Chemistry, School of Physical Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
² Department of Organic Chemistry, Faculty of Science, NCCR Chemical Biology, CH-1205 Geneva, Switzerland.

Abstract

Nucleic acids and proteins form two of the key classes of functional biomolecules. Through the ability to access specific protein-oligonucleotide conjugates, a broader range of functional molecules becomes accessible which leverages both the programmability and recognition potential of nucleic acids and the structural, chemical and functional diversity of proteins. Herein, we summarize the available conjugation strategies to access such chimeric molecules and highlight some key case study examples within the field to showcase the power and utility of such technology.

Keywords: bioconjugation; oligonucleotides; proteins.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Nucleic Acids* / chemistry
Oligonucleotides* / chemistry
Proteins

Substances

Oligonucleotides
Nucleic Acids
Proteins

Grants and funding

We thank our collaborators in the area of oligonucleotide conjugates and the funding agencies that contributed to our work (ERC, SNSF, NCCR chemical biology). This perspective is not intended to be comprehensive but instead aims to highlight recent studies, and we apologize for arbitrary omissions. Images were created with BioRender.com (accessed on 14 September 2022).