Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H2O2-Induced Oxidative Stress in Human Skin Fibroblasts

Yanbing Jiang; Shiquan You; Yongtao Zhang; Jingsha Zhao; Dongdong Wang; Dan Zhao; Meng Li; Changtao Wang

doi:10.3390/antiox11101881

Enhancing Bioactive Components of Euryale ferox with Lactobacillus curvatus to Reduce H₂O₂-Induced Oxidative Stress in Human Skin Fibroblasts

Antioxidants (Basel). 2022 Sep 23;11(10):1881. doi: 10.3390/antiox11101881.

Authors

Yanbing Jiang^{1

2}, Shiquan You^{1

2}, Yongtao Zhang^{1

2}, Jingsha Zhao^{1

2}, Dongdong Wang^{1

2}, Dan Zhao^{1

2}, Meng Li^{1

2}, Changtao Wang^{1

2}

Affiliations

¹ Beijing Key Laboratory of Plant Resource Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing 100040, China.
² Institute of Cosmetic Regulatory Science, Beijing Technology and Business University, Beijing 100040, China.

Abstract

This study investigated the effects of Lactobacillus curvatus fermentation on the oxidative stress attenuating effects of Euryale ferox on H₂O₂-induced human skin fibroblasts (HSF). The results showed that Lactobacillus curvatus fermentation (i) increases the content of the various bioactive components of Euryale ferox and is found to have smaller molecular weights of polysaccharides and polypeptides; (ii) increases the overall intracellular and extracellular antioxidant capacity of H₂O₂-induced HSF while reducing reactive oxygen species (ROS) levels. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) all showed simultaneous increases in activity. Aside from that, the Nrf2 and MAPK signaling pathways are activated to regulate downstream-associated proteins such as the Bax/Bcl-2 protein ratio, matrix metalloproteinase 1 (MMP-1) activity, and human type I collagen (COL-1). These results suggested that the fermentation of Euryale ferox with Lactobacillus curvatus enhances its antioxidant capacity and attenuates apoptosis and senescence caused by oxidative stress.

Keywords: Euryale ferox; Lactobacillus curvatus fermentation; bioactive components; oxidative stress and antiaging.

Grants and funding

This research received no external funding.