Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (Seriola dumerili) under Simulated Transport Conditions

Yuhang He; Zhengyi Fu; Shiming Dai; Gang Yu; Yunfeng Guo; Zhenhua Ma

doi:10.3390/ani12202880

Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (Seriola dumerili) under Simulated Transport Conditions

Animals (Basel). 2022 Oct 21;12(20):2880. doi: 10.3390/ani12202880.

Authors

Yuhang He^{1

2}, Zhengyi Fu^{1

2}, Shiming Dai^{1

2}, Gang Yu^{1

2}, Yunfeng Guo³, Zhenhua Ma^{1

2

4}

Affiliations

¹ Sanya Tropical Fisheries Research Institute, Sanya 572018, China.
² Hainan Provincial Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources, Sanya 572018, China.
³ Fisheries Management and Law Enforcement Service Centre, Ministry of Agriculture and Rural Affairs, Shanghai 200092, China.
⁴ Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China.

Abstract

This study investigated the effects of added eugenol on water quality and the metabolism and antioxidant capacity of the liver and gills of the greater amberjack (Seriola dumerili) during simulated transport. The juvenile fish (10.34 ± 1.33 g) were transported in sealed plastic bags containing different eugenol concentrations at a density of 24.79 kg/m³ for 8 h. The different eugenol concentrations were divided into five groups: 0 μL/mL (control group), 0.0125 μL/mL, 0.025 μL/mL, 0.0375 μL/mL, and 0.05 μL/mL, with three replicates of each. The results showed that 0.05 μL/mL of eugenol could significantly increase dissolved oxygen, but 0.025 μL/mL-0.0375 μL/mL resulted in a significant decrease in dissolved oxygen and significant increases in NH₄⁺-N and NO₂^--N. It was found that 0.05 μL/mL of eugenol caused significant up-regulation of the relative expression of CPT-1 in the liver, significant down-regulation of the relative expression of FAS and PK in the liver and gills, a significant increase in glycogen concentration, and a significant decrease in glucose concentration. This suggests that 0.05 μL/mL of eugenol could reduce the metabolic capacity of fish. In addition, 0.05 μL/mL of eugenol caused significant up-regulation of the relative expression of CAT and a significant decrease of MDA concentration in the liver. Meanwhile, the gills showed significant up-regulation of CAT relative expression, significant down-regulation of Keap1 relative expression, and a significant increase in GSH activity, resulting in a significant increase in MDA concentration when the concentration of eugenol reached or exceeded 0.025 μL/mL. This suggests that 0.05 μL/mL eugenol could improve the antioxidant capacity of fish and lipid peroxidation levels in the gills. In conclusion, the addition of 0.05 μL/mL eugenol could improve water quality, and the metabolic and antioxidant capacities of liver and gills, but it could also increase lipid peroxidation levels in the gills under transport conditions.

Keywords: Eugenol; antioxidant capacity; gill; liver; metabolism capacity; water quality.

Abstract

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