Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles

Zaruhi Hovhannisyan; Marina Timotina; Jemma Manoyan; Lilit Gabrielyan; Margarit Petrosyan; Barbara Kusznierewicz; Agnieszka Bartoszek; Claus Jacob; Mikayel Ginovyan; Karen Trchounian; Naira Sahakyan; Muhammad Jawad Nasim

doi:10.3390/antibiotics11101415

Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles

Antibiotics (Basel). 2022 Oct 14;11(10):1415. doi: 10.3390/antibiotics11101415.

Authors

Affiliations

¹ Division of Bioorganic Chemistry, School of Pharmacy, Saarland University, 66123 Saarbruecken, Germany.
² Department of Medical Biochemistry and Biotechnology, Russian-Armenian University, 0051 Yerevan, Armenia.
³ Department of Biochemistry, Microbiology and Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia.
⁴ Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdańsk University of Technology, 80-233 Gdańsk, Poland.
⁵ Research Institute of Biology, Yerevan State University, 0025 Yerevan, Armenia.

Abstract

Silver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (Ribes nigrum) leaves was determined. The main components of extract include quercetin rutinoside, quercetin hexoside, quercetin glucuronide, quercetin malonylglucoside and quercitrin. The extract was subsequently employed for the green synthesis of Ag NPs. Consequently, R. nigrum leaf extract and Ag NPs were evaluated for potential antibacterial activities against Gram-negative bacteria (Escherichia coli ATCC 25922 and kanamycin-resistant E. coli pARG-25 strains). Intriguingly, the plant extract did not show any antibacterial effect, whilst Ag NPs demonstrated significant activity against tested bacteria. Biogenic Ag NPs affect the ATPase activity and energy-dependent H⁺-fluxes in both strains of E. coli, even in the presence of N,N'-dicyclohexylcarbodiimide (DCCD). Thus, the antibacterial activity of the investigated Ag NPs can be explained by their impact on the membrane-associated properties of bacteria.

Keywords: Ribes nigrum; antimicrobial; natural products; phytochemical investigation; silver nanoparticles.

Grants and funding

This work was supported by the Science Committee of RA, in the frames of the research project No. 21AG-4D027; Basic support from Science Committee of RA, Ministry of Education, Science, Culture and Sports of RA, as well as EU Erasmus + Exchange Program by Saarland University, Germany, to Z.H.