Establishment of a human induced pluripotent stem cell line, KRIBBi006-A, from peripheral blood mononuclear cells derived from a healthy male donor

Abstract

Given the advantage of being able to be extracted by a minimally invasive method, blood is regarded as a suitable cell source for reprogramming to establish induced pluripotent stem cells (iPSCs). Therefore, iPSCs established from patient derived peripheral blood mononuclear cells (PBMCs) is widely used to develop disease modeling to elucidate disease development. Here, PBMCs from a healthy man were reprogrammed into iPSCs using the Sendai virus. The established iPSC line, KRIBBi006-A, exhibit pluripotency marker and can differentiate into the three germ layers in vitro with normal karyotype. This iPSC line is a valuable resource as a control line for stem cell research of disease models and drug screening.