A fluorescent probe MAM based on triphenylamine scaffold was synthesized. The electron donating group 4-methoxyphenyl and the electron acceptor dicyanoethylene were introduced on the triphenylamine scaffold to form a D-π-A fluorescent probe. The probe MAM exhibited the typical aggregation-induced emission (AIE) and intramolecular charge transfer (ICT) characteristics with the bright orange-red fluorescent emission in high water fraction (fw ≥ 50%) and negligible emission in low water fraction. The probe MAM could detect hydrazine (N2H4) in DMSO-tris-HCl (10 mM, pH7.4, v/v, 3:1) with high selectivity and sensitivity. The specific reaction between MAM and hydrazine and the formation of the hydrazone blocked the ICT process, and the system emitted the cyan fluorescence which could be easily observed by naked eyes. The limit of detection (LOD) was 0.196 μM (6.25 ppb), which is lower than the US Environmental Protection Agency standard (10 ppb). The test strips prepared by the probe MAM could realize the convenient and rapid detection of N2H4 solution and vapor. The application of MAM in actual water samples and cells was investigated, and the results showed that MAM could sense N2H4 in environmental and biological aspects with potential application prospects.
Keywords: AIE; Bioimaging; Fluorescent probe; Hydrazine detection; Test strips; Triphenylamine.
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