Molecularly imprinted ratiometric fluorescence (MIR-FL) nanosensors feature recognition selectivity, detection sensitivity, application universality, visualization accuracy, and device portability, and have gained popularity. However, the fluorescence intensity, nanostructure, color range, and practical application of the sensor still face severe difficulties to be solved. New strategies combined with various technologies have been developed to construct MIR-FL nanosensors for expanded applications. This Perspective highlights current resarch challenges and future prospects involving constructions and applications of MIR-FL nanosensors including dual-emission and triple-emission modes. The postimprinting mixing/modification strategies, microdevices, and multitarget detection are focused, and technology synergy, sensitivity/reproducibility improvement, application diversity/portability, etc. are proposed.