Recent advances in PCR-free nucleic acid detection for SARS-COV-2

Xiaowei Ma; Jingsong Xu; Fei Zhou; Jing Ye; Donglei Yang; Hua Wang; Pengfei Wang; Min Li

doi:10.3389/fbioe.2022.999358

Recent advances in PCR-free nucleic acid detection for SARS-COV-2

Front Bioeng Biotechnol. 2022 Oct 7:10:999358. doi: 10.3389/fbioe.2022.999358. eCollection 2022.

Authors

Xiaowei Ma¹, Jingsong Xu¹, Fei Zhou¹, Jing Ye¹, Donglei Yang¹, Hua Wang¹, Pengfei Wang¹, Min Li¹

Affiliation

¹ Department of Laboratory Medicine, Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Abstract

As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold standard method for SARS-COV-2 detection, which, however, generally requires sophisticated facilities and laboratory space, and is time consuming. This review presents recent advances in PCR-free nucleic acid detection methods for SARS-CoV-2, including emerging methods of isothermal amplification, nucleic acid enzymes, electrochemistry and CRISPR.

Keywords: CRISPR; PCR-free detection; SARS-CoV-2; isothermal amplification; nucleic acids.

Publication types

Review