Combined analysis of differentially expressed lncRNAs and miRNAs in liver tissues of high-fat fed rabbits by transcriptome sequencing

Jie Wang; Meigui Wang; Jiahao Shao; Zheliang Liu; Chong Fu; Guanhe Chen; Kaisen Zhao; Hong Li; Wenqiang Sun; Xianbo Jia; Shiyi Chen; Songjia Lai

doi:10.3389/fgene.2022.1000574

Combined analysis of differentially expressed lncRNAs and miRNAs in liver tissues of high-fat fed rabbits by transcriptome sequencing

Front Genet. 2022 Oct 7:13:1000574. doi: 10.3389/fgene.2022.1000574. eCollection 2022.

Authors

Jie Wang¹, Meigui Wang¹, Jiahao Shao¹, Zheliang Liu¹, Chong Fu¹, Guanhe Chen¹, Kaisen Zhao¹, Hong Li¹, Wenqiang Sun², Xianbo Jia², Shiyi Chen², Songjia Lai²

Affiliations

¹ College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, China.
² Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, China.

Abstract

High-fat diet could lead to a series of metabolic diseases, including obesity, and its mechanism is not clear. In this study, the rabbit individuals were fed with high-fat diet, the liver tissues were collected, high-throughput sequencing technology was used to reveal the expression of lncRNA and miRNA difference, and the molecular regulation mechanism of lncRNA-miRNA. A total of 24,615 DE lncRNAs and 52 DE miRNAs were identified, including 15 novel discovered DE miRNAs (5 upregulated and 10 downregulated). Furthermore, five miRNAs and three mRNAs were verified by qRT-PCR, and the results showed that the expression of the DE miRNAs and DE lncRNAs in the two groups was consistent with our sequencing results. GO and KEGG analyzed 7,57,139 target genes respectively, enriching the pathways related to lipid metabolism, including mucin O-glycan biosynthesis pathway, insulin resistance and glucagon signaling pathway. Moreover, 65 targeting relationships were obtained. Among them, LOC103348122/miR-450a-5p, LOC103350359/miR-450a-3p and LOC103350429/miR-148a-5p were proposed the first time. Significantly, LOC103348122/miR-450a-5p and LOC103350429/miR-148a-5p were related to lipid metabolism in the liver. This study is of great significance to the CeRNA regulatory network related to lipid metabolism in the liver of rabbits, and provides a basis for understanding hepatic steatosis in rabbits.

Keywords: HFD; lncRNA; miRNA; molecular mechanism; rabbit.