Recombinant protein G/Au nanoparticles/graphene oxide modified electrodes used as an electrochemical biosensor for Brucella Testing in milk

Hongshuo Chen; Haibin Liu; Chuanjin Cui; Wensi Zhang; Yueming Zuo

doi:10.1007/s13197-022-05544-8

Recombinant protein G/Au nanoparticles/graphene oxide modified electrodes used as an electrochemical biosensor for Brucella Testing in milk

J Food Sci Technol. 2022 Dec;59(12):4653-4662. doi: 10.1007/s13197-022-05544-8. Epub 2022 Jul 9.

Authors

Hongshuo Chen^{1

2}, Haibin Liu³, Chuanjin Cui¹, Wensi Zhang¹, Yueming Zuo²

Affiliations

¹ College of Electrical Engineering, North China University of Science and Technology, Tangshan, 063210 People's Republic of China.
² College of Engineering, Shanxi Agricultural University, Taigu, 030801 People's Republic of China.
³ College of Life Sciences, North China University of Science and Technology, Tangshan, 063210 People's Republic of China.

Abstract

In this study, a simple label-free biosensor for Brucella was constructed, which based on the screen-printed carbon electrode (SPCE) modified by Recombinant protein G/gold nanoparticles/graphene oxide (RpG/Au/GO). The impedance responses of the proposed biosensor were measured by electrochemical AC impedance method in Brucella antigen gradient concentration solutions. The results showed that the linear range of this biosensor was from 1.6 × 10² CFU/mL to 1.6 × 10⁸ CFU/mL with the minimum detection limit of 3.2 × 10² CFU/mL (S/N = 3). Moreover, the biosensor for Brucella detection possessed acceptable reproducibility with a relative standard deviation of 5.15% and acceptable stability with a relative standard deviation of 4.68%. The spiked recovery rate in actual pasteurized milk samples was more than 92%. Therefore, the developed biosensor exhibits excellent prospects in the selective quantification detection of Brucella abortus.

Supplementary information: The online version contains supplementary material available at 10.1007/s13197-022-05544-8.

Keywords: Biosensor; Brucella; Recombinant protein G; Screen-printed carbon electrode.